Evaluation of genetic diversity of GⅡ noroviruses in oyster by amplicon sequencing technology
The exploration of norovirus genetic diversity within oysters is of paramount importance in understanding the virus's epi-demic spread and devising effective prevention and control strategies.Amplicon sequencing,a next-generation sequencing(NGS)tech-nique,offers significant advantages,such as high throughput and accuracy,commonly applied in genomics,transcriptomics,and epig-enomics.This study aims to assess the viability of amplicon sequencing technology for probing norovirus diversity in oysters.To evaluate its efficacy,oyster samples were intentionally contaminated with norovirus underwent amplicon sequencing using the classical nested RT-PCR method.The analysis aimed to ascertain the viral genotype coverage and sensitivity of this approach.Results demonstrated that amplicon se-quencing successfully detected all six GⅢ noroviruses present in oysters,achieving 100％coverage.Remarkably,the technique's sensitiv-ity was underscored through the detection and sequencing analysis of oysters contaminated with varying amounts of noroviruses,confirming its ability to identify less than 50 viral particles.In summary,amplicon sequencing technology markedly enhances the accuracy and geno-typic richness of norovirus detection in oysters.
国家科技期刊平台
NSTL
万方数据
