首页|超高效液相色谱-四极杆飞行时间串联质谱结合GNPS分子网络技术检测抗生素类兽药残留

超高效液相色谱-四极杆飞行时间串联质谱结合GNPS分子网络技术检测抗生素类兽药残留

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为研究兽药残留高通量检测方法,使用超高效液相色谱-四极杆飞行时间串联质谱(ultra performance liquid chromatography-quadrupole time of flight tandem mass spectrometry,UPLC-Q-TOF-MS/MS)对畜禽基质样品以及21种兽药标准品进行检测,得到各兽药标准品的保留时间和特征碎片离子质谱图等信息,获得各目标化合物的线性方程、相关系数、线性范围以及测定底限.利用GNPS(global natural products social molecular networking)网站进一步探究了兽药的二级质谱裂解规律,对化合物进行结构解析和确证,发现同一种类的兽药分子裂解规律存在相似性,可以借助兽药分子的二级质谱裂解碎片实现畜禽肉中兽药残留的快速高通量筛查.基于UPLC-Q-TOF-MS/MS及GNPS分子网络技术,在24个猪肉、鱼肉样品中检出10种抗生素阳性,其中有3种不包含在已建立的兽药标准品信息库中.结果表明,相比于常规检测方法.UPLC-Q-TOF-MS/MS结合GNPS分子网络技术可以快速、简便地实现畜禽肉中兽药残留检测,且能发现未知兽药.
Detection method of antibiotic veterinary drug residues based on ultra performance liquid chromatography-quadrupole time of flight tandem mass spectrometry combined with GNPS molecular network
To investigate the high throughput detection method of veterinary drug residues,ultra performance liquid chromatography-quadrupole time of flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS)was used to detect livestock and poultry matrix samples and 21 standard veterinary drugs.The retention time and characteristic fragment ion mass spectra of each standard veterinary drug were ob-tained.The linear equation,correlation coefficient,linear range,and limit of detection were obtained.Based on the GNPS(global natural products social molecular networking)website,the secondary mass spectrometry cleavage of veterinary drugs was further explored,and the structure of the compound was analyzed and confirmed.Results showed that the cleavage pattern of the same type of veterinary drug mole-cule was similar,and the rapid high throughput screening of veterinary drug residues in livestock and poultry meat could be realized based on the secondary mass spectrometry lysis fragments of veterinary drug molecule.According to UPLC-Q-TOF MS/MS and GNPS molecular network technology,10 drugs were detected positive in 24 matrix samples of pork and fish,and 3 positive drugs were not included in the established standard veterinary drug information database.Indicating that compared with the conventional detection methods,UPLC-Q-TOF MS/MS combined with GNPS molecular network technology can realize the identification of veterinary drug residues in livestock and poul-try meat quickly and accurately,and it can also find unknown veterinary drugs.

veterinary drug residuesmass spectrometry fragmentation patternGNN PS molecular networkultra performance liquid chro-matography-quadrupole time of flight tandem mass spectrometry

王伟、任丽秋、方志娟、胡文涛、张芬、张雪婧、赵紫薇、马家辉、李晓芹、成向荣

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苏州市食品检验检测中心,江苏苏州,215100

江苏省市场监管重点实验室(食品安全快检技术与产品评价),江苏苏州,215100

江南大学食品学院,江苏无锡,214122

兽药残留 质谱裂解规律 GNPS分子网络 超高效液相色谱-四极杆飞行时间串联质谱

2024

食品与发酵工业
中国食品发酵工业研究院 全国食品与发酵工业信息中心

食品与发酵工业

CSTPCD北大核心
影响因子:0.761
ISSN:0253-990X
年,卷(期):2024.50(24)