Multiple studies have demonstrated that using Yarrowia lipolytica as a cell factory for cordycepin biosynthesis is an effective way to produce cordycepin.To obtain the separation and purification process,this study utilized macroporous adsorption resins with differ-ent specifications to purify cordycepin from recombinant Y.lipolytica fermentation broth by static and dynamic purification.Results showed that the recovery rate and purity of cordycepin could reach 87.67％and 65.82％by using NKA-Ⅱ macroporous adsorption resin with a cordycepin concentration of 3 mg/mL and pH of 9,loading flow rate of 3 BV/h,loading volume of 10 BV,wash flow rate of 2 BV/h,wash volume of 3 BV,and 30％ethanol for elution solution,with a flow rate of 3 BV/h and volume of 15 BV.This study aims to provide data references for the future large-scale production and preparation of cordycepin using recombinant Y.lipolytica.
关键词
虫草素/解脂耶氏酵母/分离纯化/大孔吸附树脂/甘蔗糖蜜
Key words
cordycepin/Yarrowia lipolytica/separation and purification/macroporous adsorption resin/molasses
维普
万方数据