分离纯化重组解脂耶氏酵母发酵液中虫草素的研究
Isolation and purification of cordycepin from the fermentation of engineering Yarrowia lipolytica
宋丽萍 1段希宇 2王翀 1卢向阳 1田云1
作者信息
- 1. 湖南农业大学生物科学技术学院,湖南长沙,410128
- 2. 湖南农业大学生物科学技术学院,湖南长沙,410128;沅江市芦小妹食品有限公司,湖南益阳,413000
- 折叠
摘要
多项研究证明以解脂耶氏酵母作为虫草素生物合成的细胞工厂,是获得虫草素的有效途径.为获得下游虫草素分离纯化工艺,该研究采用不同规格的大孔吸附树脂对重组解脂耶氏酵母发酵液中的虫草素进行静态、动态纯化.结果显示,以NKA-Ⅱ大孔吸附树脂作为吸附材料,在虫草素质量浓度为3 mg/mL、pH值为9的上样液以上样流速为3 BV/h、上样体积为10 BV、洗杂流速为2 BV/h、洗杂体积为3 BV、洗脱剂为体积分数为30％乙醇、洗脱流速为3 BV/h、洗脱体积为15 BV时,发酵液中虫草素回收率可达到87.67％,纯度达65.82％.该研究为今后利用重组解脂耶氏酵母大规模生产制备虫草素提供数据参考.
Abstract
Multiple studies have demonstrated that using Yarrowia lipolytica as a cell factory for cordycepin biosynthesis is an effective way to produce cordycepin.To obtain the separation and purification process,this study utilized macroporous adsorption resins with differ-ent specifications to purify cordycepin from recombinant Y.lipolytica fermentation broth by static and dynamic purification.Results showed that the recovery rate and purity of cordycepin could reach 87.67％and 65.82％by using NKA-Ⅱ macroporous adsorption resin with a cordycepin concentration of 3 mg/mL and pH of 9,loading flow rate of 3 BV/h,loading volume of 10 BV,wash flow rate of 2 BV/h,wash volume of 3 BV,and 30％ethanol for elution solution,with a flow rate of 3 BV/h and volume of 15 BV.This study aims to provide data references for the future large-scale production and preparation of cordycepin using recombinant Y.lipolytica.
关键词
虫草素/解脂耶氏酵母/分离纯化/大孔吸附树脂/甘蔗糖蜜Key words
cordycepin/Yarrowia lipolytica/separation and purification/macroporous adsorption resin/molasses引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
维普
万方数据