Expression and characterization of the lipase LipTL from Thermomyces lanuginosus and its application in oil degumming
Enzymatic degumming technology has great potential application in oil refining industry.Phospholipases are the major en-zymes that applied in oil degumming.However,the existing phospholipases are unable to meet the requirements of industrial degumming processes in terms of substrate selectivity and temperature stability,which limits the large-scale application of phospholipases in the oil re-fining industry.Researches have revealed that the lipase(LipTL)from Thermomyces lanuginosus has ability of hydrolyzing phospholipids.In this study,this lipase LipTL gene was heterologously expressed in Escherichia coli,then the recombinant LipTL was purified and char-acterized,and its application in oil degumming was also studied.Sequence alignment exhibited that LipTL showed high similarity with phospholipase A1;SDS-PAGE revealed that the molecular weight of LipTL was approximately 30 kDa;The optimal temperature and pH for LipTL were 55 ℃ and 9.0 respectively,and it could maintain over 90％relative activity after 2 hours of treatment at 55 ℃.After 6 hours of degumming reaction,LipTL reduced the residual phosphorus content of soybean oil and peanut oil to 5.2 mg/kg and 4.9 mg/kg,re-spectively.This study confirms that the lipase LipTL can be applied to oil degumming,which not only provides a new enzyme source for enzymatic degumming,but also lays a good foundation for the subsequent modification of this enzyme.
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