Identification of ZjERF098 and investigation of its enhancing effect on winter jujube resistance to black spot rot
ZjERF098 was cloned and characterized to clarify its molecular properties and to verify the inhibitory effect of transient overexpression of ZjERF098 on black spot rot of jujube,so as to lay the foundation for the research on the mechanism of resistance to dis-ease of jujube fruit.The phylogenetic tree construction,homologous sequence comparison,structural domain analysis,protein structure prediction and promoter cis-acting element analysis were performed on ZjERF098.Several recombinant vectors such as pEAQ-ZjERF098 were constructed to analyze the subcellular localization and activation of transcriptional activity of ZjERF098 in tobacco,and its transient overexpression effect in jujube fruit.The results showed that the open reading frame of ZjERF098 comprises 426 base pairs(bp),enco-ding 141 amino acids.It shared the highest sequence similarity with the AtERF098 sequence in Arabidopsis thaliana,belonging to the ERF transcription factor subfamily.ZjERF098 had only one AP2 domain and lacked a signal peptide and transmembrane domains.The promoter region contained response elements related to plant defense responses,such as abscisic acid responsive elements and jasmonic acid respon-sive elements.ZjERF098 exhibited transcriptional activation activity,primarily localizing in the cell nucleus,with GFP fluorescence sig-nals also observed on the cytoplasmic membrane.Simultaneously,transient overexpression of ZjERF098 enhanced the resistance of jujube fruits to black spot rot,significantly reducing disease incidence and lesion diameter.These indicated that ZjERF098 play a crucial role as a transcription factor in enhancing the resistance of jujube fruits to black spot rot.
万方数据
维普
