自然发酵虾酱中屎肠球菌的分离、鉴定及体外益生特性分析
Isolation and identification of Enterococcus faecium in naturally fermented shrimp sauce and in vitro probiotics properties
王鑫 1江宁 2张煜 1孙荣雪 2王成 2杨慧晶 3马艳弘 2梁丽雅4
作者信息
- 1. 天津农学院食品科学与生物工程学院,天津,300384;江苏省农业科学院农产品加工研究所,江苏南京,210014
- 2. 江苏省农业科学院农产品加工研究所,江苏南京,210014
- 3. 淮安生物健康产业创新研发中心,江苏淮安,223001
- 4. 天津农学院食品科学与生物工程学院,天津,300384
- 折叠
摘要
从自然发酵的小龙虾酱中筛选出8株产酸菌,通过生理生化试验和16S rDNA序列分析对菌种进行鉴定,并根据发酵特性分析选取产酸能力最高的菌株LX12-2和LX18-9进行抑菌、抗氧化、体外益生特性分析及安全性评价.结果表明,有7株菌为乳酸菌,隶属肠球菌属,其中5株为粪肠球菌(Enterococcus faecalis)、2株为屎肠球菌(Enterococcus faecium);屎肠球菌LX18-9的产酸能力最高,具有广谱的抗菌活性和较高的抗氧化能力.其对单增李斯特菌生长的抑制作用最强,抑菌圈直径可达20.85 mm,对DPPH自由基的清除率达76.77％;该菌株对pH 2.0的酸性条件、0.5％胆盐以及人工胃肠液均具有良好耐受性,且不产组胺,无溶血性,安全性高.综上,该研究筛选的屎肠球菌LX18-9安全性高、且具有良好的抗菌、抗氧化及益生特性,在饲料发酵生产与水产养殖益生产品的开发中具有潜在应用价值.
Abstract
Eight strains of acid-producing bacteria were selected from naturally fermented lobster shells,and identified through physi-ological and biochemical tests and 16S rDNA sequence analysis.Based on fermentation characteristics analysis,the highest acid-producing strains LX12-2 and LX18-9 were selected for antibacterial,antioxidant,and in vitro probiotic characteristics analysis and safety evalua-tion.Results showed that there were 7 strains of lactic acid bacteria belonging to the genus Enterococcus,including 5 strains of E.faecalis and 2 strains of Enterococcus faecium.Enterococcus faecalis LX18-9 had the highest acid production ability,broad-spectrum antibacterial activity,and high antioxidant capacity.It had the strongest inhibitory effect on the growth of Listeria monocytogenes,with a diameter of 21.85 mm and a scavenging rate of 76.77％on DPPH free radicals.This strain had a good tolerance to pH 2.0 acid conditions,0.5％bile salt,and artificial gastrointestinal fluid,no histamine production,no hemolysis,and a high safety profile.In summary,the E.faeca-lis LX18-9 screened in this study had high safety and good antibacterial,antioxidant,and probiotic properties,which had potential appli-cation value in feed fermentation production and the development of probiotic products in aquaculture.
关键词
小龙虾/屎肠球菌/分离鉴定/益生特性/安全性Key words
crayfish/Enterococcus faecalis/isolation and identification/probiotic characteristics/security引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
维普
万方数据