Identification of jujube(Ziziphus jujuba Mill.)honey protein by gel-based and gel-free proteomics
Protein is a relatively stable macromolecular substance existing in honey,and the research on honey protein is of great sig-nificance in exploring honey biological activity and screen marker ingredients.Jujube(Ziziphus jujuba Mill.)honey,a characteristic monofloral honey in China,was selected to investigate its protein components by gel-based and gel-free proteomics.Results showed that 216 bee-derived and 595 plant-derived proteins were identified in the collected samples.Among these proteins,24 bee-derived proteins were first reported in jujube honey,including MAGUK p55 subfamily member 6 isoform X1,chitin-binding type-2 domain-containing pro-tein,peptidase S1 domain-containing protein,and so on.Notably,by BLAST analysis,11 proteins,including metalloendoproteinase 2-MMP-like,FAS1 domain-containing protein,peptidase S1 domain-containing protein,etc.could be considered as potential markers for ju-jube honey.GO analysis revealed these bee-derived and plant-derived proteins mainly were involved in the processes of carbohydrate meta-bolic and proteolysis,and were associated with the functions of ATP binding and guanosine triphosphate binding.The research comprehen-sively investigated the jujube protein components,and provided a theoretical reference for investigating the protein bioactive components and screening unique protein makers in jujube honey.
万方数据
维普
