Exploration of Monascus disturbance on high-temperature Daqu and their manufacturing process
This study concentrated high-temperature Daqu,examining the distinctions in physicochemical properties and metabolic components between conventional(T)and shelf(J)fermentation pattern,as well as the effect of Monascus strains disturbance by using the multiphase detection techniques.The finding revealed that the J patterns enhanced fermenting power and the richness and content of al-cohols,while reducing esterification capability.Disturbances from Monascus strain increased the levels of total volatile and ester compo-nents in Daqu,modifying the composition profile.The impact of the fermentation pattern on contribution characteristics varied,with dis-turbance notably improving the physicochemical properties and metabolic component contents in the T pattern.Specifically,strain H26 was identified as contributing significantly to the tetramethyl pyrazine content,reaching as high as 0.38 mg/kg.The isolated strains enhanced the presence of functional microorganism such as Saccharopolyspora,Bacillus,and Thermoactinomyces in Daqu.Investigation into the rela-tionship between functional microorganism and the physicochemical properties and metabolic components indicated that bacteria generally had a negative correlations with volatile components,whereas genera like Kroppenstedtia and Thermoactinomyces showed positive correla-tion with various non-volatile components.Enzyme expression analysis annotated using KEGG highlighted metabolic pathway characteris-tics,with EC:2.6.1.1 being highly expressed in the J pattern,contributing to an increase phenylethyl alcohol content.Conversely,the T pattern exhibited high expression of EC:1.4.1.3,EC:1.4.1.4,and EC:6.3.4.14 in related metabolic pathways,enhancing the content of tetramethyl pyrazine and ester components.Therefore,choice of the fermentation pattern and the specific Monascus strains significantly affect disturbance characteristics.These results offer crucial insight the application of biological disturbance technology for the targeted con-struction of metabolites.
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维普
