为探究不同品种芜菁冻干片中挥发性有机物(volatile organic compounds,VOCs)的差异,采用顶空-固相微萃取-气相色谱-质谱(headspace solid phase microextraction gas chromatography-mass spectrometry,HS-SPME-GC-MS)和顶空-气相色谱-离子迁移谱(headspace gas chromatography-ion mobility spectrometry,HS-GC-IMS)对紫色、黄色和白色3种芜菁冻干片的V OCs进行分析,并结合主成分分析(principal component analysis,PCA)和偏最小二乘判别法(partial least squares-discriminant analysis,PLS-DA)等化学计量法探究不同品种芜菁冻干片挥发性成分的差异.结果表明,通过HS-SPME-GC-MS共解析出96种VOCs,包括醛类、醇类、酮类、含硫化合物、酯类、酸类等化合物,其中含硫化合物和酯类为芜菁冻干片中相对含量最高的化合物种类;HS-GC-IMS共解析出94种VOCs,包括醛类、酯类、酮类及含硫化合物等挥发性成分.HS-SPME-GC-MS和HS-GC-IMS检出的挥发性物质种类和含量存在差异,共有VOCs有15种,二者结果互为补充,结合使用可以较全面系统地表征芜菁冻干片的挥发性成分.PCA和PLS-DA结果表明,2种方法均能够有效区分3种芜菁冻干片.通过变量投影重要度分别筛选了 59种和23种差异VOCs,该结果可为芜菁冻干片VOCs的差异分析提供参考方法.
Analysis of volatile components in turnip freeze-dried slices based on headspace solid phase microextraction gas chromatography-mass spectrometry and headspace gas chromatography-ion mobility spectrometry combined with chemometrics
To investigate the differences of volatile organic compounds(VOCs)in turnip freeze-dried slices with different varieties,headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS)and headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS)were applied to analyze the VOCs of purple,yellow,and white turnip freeze-dried slices,and the differences of volatile components freeze-dried slices from different varieties were investigated using the chemometric methods,such as principal component analysis(PCA)and partial least squares-discriminant analysis(PLS-DA).Results showed that a total of 96 VOCs,including aldehydes,alcohols,ketones,sulfur-containing compounds,esters,acids,and other compounds,were resolved by HS-SPME-GC-MS,in which sulfur-containing compounds and esters were the highest relative content of compounds in the freeze-dried slices of tur-nip,and a total of 94 VOCs,including aldehydes,esters,ketones and sulfur-containing compounds,and other substances,were resolved by HS-GC-IMS.HS-SPME-GC-MS and HS-GC-IMS detected different types and contents of volatiles,and 15 VOCs were detected in com-mon,and the two results could be used in combination for a more comprehensive and systematic characterization of the volatile components of turnip freeze-dried slices.Combination of the two methods could comprehensively characterize the differences in volatile compositions of turnip freeze-dried slices with different varieties.The results of PCA and PLS-DA showed that both assays were able to effectively differen-tiate the three types of turnip freeze-dried slices.59 and 23 differential VOCs were screened by variable importance in projection,respec-tively,to provide a reference method for the differential analysis of VOCs in turnip freeze-dried slices.
turnip freeze-dried slicesvolatile organic compoundsheadspace-solid phase microextraction-gas chromatography-mass spectrometryheadspace gas chromatography-ion mobility spectrometryvariable importance in projection
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