The cyclic soaking process,hailed as a"green technology",faces challenges in industrial application due to the complex microbial ecology within factory environments.To combat the intrusion of biogenic amines-producing microorganisms during the cyclic soa-king process,achieving"zero growth"of biogenic amines and multi-batch cycling,this study first elucidated that the metabolism of bio-genic amines during cyclic soaking was a result of microbial interactions.Correlation analysis revealed significant associations between Lac-tiplantibacillus plantarum,Pediococcus damnosus,Latilactobacillus curvatus,Loigolactobacillus coryniformis,Levilactobacillus brevis,and the reduction of biogenic amines in rice seriflux.Based on predictive results,the core microbial community in rice seriflux was selected and constructed,demonstrating a 55.21％inhibition of biogenic amines in verification experiments.Finally,a synthetic microbial consor-tium-based cyclic soaking process was developed,achieving"zero growth"of biogenic amines in simulated factory cyclic soaking process experiments.Experimental results indicated that the constructed communities of L.plantaurm,L.brevis,and L.curvatus exhibited strong resistance to intrusion by biogenic amines-producing microorganisms,thereby creating feasibility for the industrial application of the cyclic soaking process.
biogenic aminesmicrobial community constructionHuangjiucyclic soaking
维普
万方数据
