首页|益生菌发酵枸杞汁及其体外降血糖与抗氧化活性研究

益生菌发酵枸杞汁及其体外降血糖与抗氧化活性研究

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为评价发酵对枸杞汁体外降血糖及抗氧化活性的影响,通过鼠李糖乳酪杆菌、嗜酸乳杆菌、植物乳植杆菌、副干酪乳酪杆菌对枸杞汁的发酵性能、体外降血糖活性优选出发酵枸杞汁的最适菌株,采用响应面法对枸杞汁的发酵工艺进行优化.结果表明,副干酪乳酪杆菌表现出更好的耐酸、耐胆盐、发酵性能及较高的α-淀粉酶及α-葡萄糖苷酶抑制性;最佳发酵工艺为发酵时间9 h,接种量5%,发酵温度37 ℃.与未发酵枸杞汁相比,多酚含量增加5.66%,总黄酮含量显著提高31.81%,DPPH自由基和羟自由基的清除率最高可达到92.3%、95.84%,ABTS阳离子自由基的清除率显著提高42.74%,同时α-葡萄糖苷酶抑制率也显著提高30.23%.益生菌发酵在一定程度上提升了枸杞汁的体外抗氧化和降血糖活性,对后续研究具有降血糖功能的发酵枸杞食品提供了理论基础.
Study on probiotic fermented goji berry juice and its in vitro hypoglycemic and antioxidant activities
To evaluate the effect of fermentation on the in vitro hypoglycemic and antioxidant activities of goji berry juice,the most suitable strains for fermentation of goji berry juice were preferably selected through the fermentation performance and in vitro hypoglycemic activities of Lacticaseibacillus rhamnosus,Lactobacillus acidophilus,Lactiplantibacillus plantarum,and Lacticaseibacillus paracasei on goji berry juice.The fermentation process of goji berry juice was optimized by using response surface methodology.Results showed that L.para-casei showed better acid resistance,bile salt resistance,fermentation performance,and higher α-amylase and α-glucosidase inhibition.The optimal fermentation process was 9 h of fermentation time,5% inoculum,and a fermentation temperature of 37 ℃.Compared with unfermented wolfberry juice,the polyphenol content increased by 5.66%,the total flavonoid content increased significantly by 31.81%,the scavenging rate of DPPH free radicals and hydroxyl radicals could reach up to 92.3% and 95.84%,and the scavenging rate of ABTS cationic radicals was significantly increased by 42.74%,as well as the inhibition rate of α-glucosidase was also significantly increased by 30.23%.Probiotic fermentation enhanced the in vitro antioxidant and hypoglycemic activities of goji berry juice to a certain extent,which provided a theoretical basis for the subsequent study of fermented goji berry food with hypoglycemic function.

goji berry juiceprobioticfermentation processantioxidanthypoglycemic

漆莹、魏晓博、马铖、刘慧燕、方海田

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宁夏大学食品科学与工程学院,宁夏食品微生物应用技术与安全控制重点实验室,宁夏银川,750021

宁夏三十六度生物科技有限公司,宁夏银川,750021

枸杞汁 益生菌 发酵工艺 抗氧化 降血糖

2025

食品与发酵工业
中国食品发酵工业研究院 全国食品与发酵工业信息中心

食品与发酵工业

影响因子:0.761
ISSN:0253-990X
年,卷(期):2025.51(9)