Optimization of culture conditions for compound starter of pickles
To obtain the better formula of culture conditions for compound starter of pickles,culture conditions were studied by orthogonal design,quadratic polynomial stepwise regression and etc.Response surfaces are drawn according to the mathematical model among factors,and it was discussed that the impact of various factors on the two lactic acid bacteria mixed bacterial culture.The results showed,with 16.3％ of enzymatic hydrolyzate of skim milk,0.9％of the glucose and 1.9％ of VC addition,after 30 ℃ for 6 h,the number of bacteria reached 1.6×1010CFU/mL.The data were closed to theoretical values,and the effect was better than the tradi tional MRS lactic acid bacteria culture medium.The order of factors affecting the cell number were enzyme solution＞glucose content and ＞VC content
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