Fast separation and purification of bovine immunoglobulin using magnetic nanoparticles
The magnetic nanoparticles (MNPs) were used to quickly separate and purify bovine immunoglobulin (IgG) from cattle blood.The effects of the pH of the binding buffer (Sodium phosphate buffer) and the concentration of NaCl,the adsorption time on the purity and recovery of immunoglobulin (IgG) were investigated to determine the optimum conditions for separation and purification of the protein by means of response surface methodology.The purity of IgG first increased and then decreased quickly with increasing pH values,but the protein recovery was always increased,this indicated that the optimum pH of binding buffer was 6.65.The purity and recovery of IgG first increased and then decreased with an increase with NaCl concentration,the maximum of IgG adsorption was observed with the presence of 0.5 mol/L NaCl.The experimental results also showed that under the optimal adsorption conditions,the optimum static adsorption time was 2 h.The recovery and purity of extracted IgG are 90.33％ and 83.75％ under optimal conditions,respectively.
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维普
