Optimization of aqueous enzymatic method extraction of lycium seed oil by quadratic orthogonal rotation design
The extraction of lycium seed oil by aqueous enzymatic method was adopted.The experimental values were firstly selected by single-factor test and a quadratic orthogonal rotation combination design was chosen based on the results of single factor test in order to study the effects of three main technological conditions,including ratio of soild to solvent,dosage of Viscozyme L and enzymolysis time on yield of oil from lycium seed.Results showed that the influence of investigated facors on yield of lycium seed oil followed the decreasing order:ratio of soild to solvent＞dosage of Viscozyme L＞ enzymolysis time and a maximum yield of 87.6％ was achieved under the optimal extraction:ratio of soild to solvent 3.6 ∶ 1(V ∶ m),dosage of Viscozyme L 3.0％ and enzymolysis time 3 h.
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