首页|蓝莓花色苷对鸡胚成纤维细胞系生长的影响

蓝莓花色苷对鸡胚成纤维细胞系生长的影响

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以花色苷含量为21.34%的蓝莓花色苷粉末为原料,采用MTT法、显微镜观察法,同时利用细胞计数仪测定细胞活率,3种方法共同检测蓝莓花色苷作用于鸡胚成纤维细胞系的细胞毒性,以完成细胞试验模型的建立.结果表明:50~100 μg/mL的蓝莓花色苷可以促进鸡胚成纤维细胞生长,高于100 μg/mL的蓝莓花色苷,对细胞生长产生抑制作用.该结果可为进一步拓展花色苷功能性研究,以及以鸡胚成纤维细胞为模型的生物功能性研究提供理论依据.
Effect of anthocyanins from blueberry on primary chicken embryonic fibroblast
The effect of blueberry anthocyanins on the growth of primary chicken embryonic was investigated fibroblast in this study.21.34% of anthocyanins was used to The situation of cell growth was evaluate by optical microscope,cell counter,and MTT assays.We observed that 50-100 μg/mL was the optimal concentration,which had no cytotoxicity to primary chicken embryonic fibroblast.It would inhibit cell growth when the concentration was more than 100 μg/mL.The results of this study may provide the basis for further development of anthocyanins functional studies and give helpful information for preventing the virus which used primary chicken embryonic fibroblast as cell model.

blueberry anthocyaninsprimary chicken embryonic fibroblastMTT assay

王丹、黄紫藤、马越、赵晓燕

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北京市农林科学院蔬菜研究中心,北京100097

农业部华北地区园艺作物生物学与种质创制重点实验室,北京100097

农业部都市农业(北方)重点实验室,北京 100097

北京农学院食品科学与工程学院,北京102206

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蓝莓花色苷 鸡胚成纤维细胞系 MTT法

北京市科技新星计划现代农业产业技术体系建设专项资金资助

Z131105000413023CARS-25

2016

食品与机械
长沙理工大学

食品与机械

CSTPCDCSCD北大核心
影响因子:0.89
ISSN:1003-5788
年,卷(期):2016.32(8)
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