Screening and identification of Lactic Acid Bacteria with acid and bile tolerance
In order to screen human probiotics with the acid and bile resistant abilities, infant stool diluents were spread on five different selective ager plates including TPY, BHI, MRS and SL in this study.42 strains of lactic acid bacteria with certain bile-tolerance were isolated by selective ager media for lactobacilli, supplemented with bile salt (0.2%) and bromcresol purple as an acid indicator.3 strains of lactic acid bacteria with high acid tolerance were further selected from 42 strainsaccording to biomass of their cultured in the MRS liquid medium with pH 5.0, pH 4.0 and pH 3.5 respectively.Then the viability of these 3 strains in MRS medium with pH 3.0 was measured by plate colony count technique, resulting in one of them with the highest acid-and bile salt-tolerance.Then this strain was subsequently identified using 16S rRNA-based molecular biological approaches.The strain was molecularly identified to be.Lactobacillus plantarum.The strain could be used as a potential probiotic strain for late digging its probiotic function.
国家科技期刊平台
NSTL
万方数据
维普
