首页|扁桃果皮熊果酸提取工艺优化及体外抗氧化活性研究

扁桃果皮熊果酸提取工艺优化及体外抗氧化活性研究

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在单因素试验的基础上,采用正交试验优化扁桃果皮中熊果酸的提取工艺参数,并通过还原能力、DPPH·、·OH 及小鼠红细胞氧化溶血试验进行抗氧化活性评价.结果表明:扁桃果皮中熊果酸最优提取工艺参数为乙醇浓度65%、提取温度80 ℃、料液比1︰20 (g/mL)、提取时间1.5 h,该条件下,熊果酸提取率为2.89%.熊果酸浓度为0.1 mg/mL 时,扁桃果皮熊果酸具有良好的还原能力,对DPPH·和·OH 的最大清除率分别为87.87%,62.11%;熊果酸浓度为0.6 mg/mL 时,对小鼠红细胞氧化溶血抑制率为97.83%.
Extraction and antioxidant activity of ursolic acid from AmygdaIus communis L.pee l
Based on the results of single-factor experiments,orthogo-nal test of ursolic acid from Amygdalus communis L.peel were car-ried out to determine the optimum process parameters.The antioxidant activities were evaluated by the reducing power,DPPH free radical,hydroxyl free radical and hemolysis of mice red blood cells.The optimum extraction parameters were determined as follows:alcohol concentration 65%,extraction temperature 80 ℃;solid-to-solvent ratio 1︰20 (g/mL);extraction time 1.5 h.Under these conditions,the experimental yield of ursolic acid was 2.89%. The concentration of ursolic acid was 0.1 mg/ml,ursolic acid of Amygdalus communis L.peel displayed a strong antioxidant activity,the percentage scavenging of DPPH free radical and ·OH were 87.87% and 62.11%,respectively.The concentration of ursolic acid was 0.6 mg/mL,the oxidation of mice red blood cell hemolysis inhibition rate was 97.83%.

amygdalus communisursolic acidextractionantioxi-dant activity

张俊、李进、吕海英、马雪

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新疆特殊环境物种保护与调控生物学实验室,新疆 乌鲁木齐 830054

新疆师范大学干旱区植物逆境生物学重点实验室,新疆 乌鲁木齐 830054

新疆师范大学生命科学学院,新疆 乌鲁木齐 830054

扁桃 熊果酸 提取 抗氧化

新疆师范大学"十三五"重点学科招标项目

17SDKD0203

2018

10.13652/j.issn.1003-5788.2018.02.033

食品与机械
长沙理工大学

食品与机械

CSTPCD北大核心
影响因子：0.89
ISSN：1003-5788
年,卷(期)：2018.34(2)
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