首页|净化柱结合超高效液相色谱—串联质谱检测动物源性食品中16种喹诺酮药物

净化柱结合超高效液相色谱—串联质谱检测动物源性食品中16种喹诺酮药物

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
目的:加强动物源性食品安全检测市场监管。方法:动物源性食品样品使用80％乙腈(含0。2％甲酸)提取，净化柱(Speedy Prep®-Quino 1)净化后，利用超高效液相色谱—串联质谱检测16种喹诺酮类药物残留。结果:16种喹诺酮类药物的线性范围为1。6～40。0 μg/kg，相关系数r≥0。996 1，检出限为0。14～0。80 μg/kg，定量限为0。47～2。68 μg/kg。在净化柱前处理后7个基质加标回收率为62％～112％，相对标准偏差为0。9％～18。7％。结论:该方法具有检测速度快、灵敏度高的特点，适用于羊肉、鸭肉、牛肉、鱼肉、鸡蛋、猪腰、鸭皮等动物源性食品。
Establishment and application of clean-up combined with ultra-high performance liquid chromatography tandem mass spectrometry for the detection of 16 quinolones in animal-derived food
Objective:A method for simultaneous detection of 16 quinolones in food of animal origin was developed byclean-up column pretreatment combined with ultra high performance liquid chromatography-tandem mass spectrometry.Methods:Food samples of animal origin were extracted using 80％acetonitrile(containing 0.2％formic acid).After purification on a Speedy Prep®-Quino 1 column,16 quinolone residues were detected by ultra-high performance liquid chromatography tandem mass spectrometry.Results:The results showed that the linear range of 16 quinolones was 1.6～40.0 μg/kg,the correlation coefficient r≥0.996 1,the limits of detection were 0.14～0.80 μg/kg,and the limits of quantification were 0.47～2.68 μg/kg.The recoveries of seven matrix after pretreatment were 62％～112％,and the relative standard deviations were 0.9％～18.7％.Conclusion:The method has the characteristics of fast detection speed and high sensitivity,and can be applied to animal derived food such as mutton,duck,beef,fish,eggs,pig kidney,duck skin.

animal-derived foodquinolonespretreatment of clean-up columnultra-high performance liquid chromatography tandem mass spectrometry

余婷婷、梁松、黄文慧、何洁、严义勇

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深圳市易瑞生物技术股份有限公司,广东深圳 518101

深圳职业技术学院集成电路关键材料研究院,广东深圳 518055

动物源性食品 喹诺酮 净化柱前处理 超高效液相色谱—串联质谱

广东省粤港湾联合创新领域项目资助深圳市技术攻关重点项目

2021A0505080003JSGG20191115141601721

2024

10.13652/j.spjx.1003.5788.2023.80073

食品与机械
长沙理工大学

食品与机械

CSTPCD北大核心
影响因子：0.89
ISSN：1003-5788
年,卷(期)：2024.40(1)
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