净化柱结合超高效液相色谱—串联质谱检测动物源性食品中16种喹诺酮药物
Establishment and application of clean-up combined with ultra-high performance liquid chromatography tandem mass spectrometry for the detection of 16 quinolones in animal-derived food
余婷婷 1梁松 1黄文慧 1何洁 2严义勇1
作者信息
- 1. 深圳市易瑞生物技术股份有限公司,广东深圳 518101
- 2. 深圳市易瑞生物技术股份有限公司,广东深圳 518101;深圳职业技术学院集成电路关键材料研究院,广东深圳 518055
- 折叠
摘要
目的:加强动物源性食品安全检测市场监管.方法:动物源性食品样品使用80％乙腈(含0.2％甲酸)提取,净化柱(Speedy Prep®-Quino 1)净化后,利用超高效液相色谱—串联质谱检测16种喹诺酮类药物残留.结果:16种喹诺酮类药物的线性范围为1.6～40.0 μg/kg,相关系数r≥0.996 1,检出限为0.14～0.80 μg/kg,定量限为0.47～2.68 μg/kg.在净化柱前处理后7个基质加标回收率为62％～112％,相对标准偏差为0.9％～18.7％.结论:该方法具有检测速度快、灵敏度高的特点,适用于羊肉、鸭肉、牛肉、鱼肉、鸡蛋、猪腰、鸭皮等动物源性食品.
Abstract
Objective:A method for simultaneous detection of 16 quinolones in food of animal origin was developed byclean-up column pretreatment combined with ultra high performance liquid chromatography-tandem mass spectrometry.Methods:Food samples of animal origin were extracted using 80％acetonitrile(containing 0.2％formic acid).After purification on a Speedy Prep®-Quino 1 column,16 quinolone residues were detected by ultra-high performance liquid chromatography tandem mass spectrometry.Results:The results showed that the linear range of 16 quinolones was 1.6～40.0 μg/kg,the correlation coefficient r≥0.996 1,the limits of detection were 0.14～0.80 μg/kg,and the limits of quantification were 0.47～2.68 μg/kg.The recoveries of seven matrix after pretreatment were 62％～112％,and the relative standard deviations were 0.9％～18.7％.Conclusion:The method has the characteristics of fast detection speed and high sensitivity,and can be applied to animal derived food such as mutton,duck,beef,fish,eggs,pig kidney,duck skin.
关键词
动物源性食品/喹诺酮/净化柱前处理/超高效液相色谱—串联质谱Key words
animal-derived food/quinolones/pretreatment of clean-up column/ultra-high performance liquid chromatography tandem mass spectrometry引用本文复制引用
基金项目
广东省粤港湾联合创新领域项目(2021A0505080003)
资助深圳市技术攻关重点项目(JSGG20191115141601721)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据