Study on the technology of ultrasonic assisted combined extraction of saponins and polysaccharides from Polygonatum sibiricum and its hypoglycemic activity
Objective:With the Polygonatum sibiricum as raw material,to develop a process for the combined extraction of Polygonatum sibiricum sponins(PSSs)and polysaccharides(PSPs),and the inhibitory activities of the extract and their complexes(PSSs-PSPs)on α-glucosidase and a-amylase were investigated.Methods:PSSs and PSPs were performed using ultrasound combined with enzymatic hydrolysis.The effects of solid-liquid ratio,ultrasonic time,and ultrasonic temperature on the yield of PSSs,the types of multi-enzymes,the proportion of complex enzymes,enzymolysis time,enzyme dosage,solid-liquid ratio,hot water extraction time,and hot water extraction temperature on the yield of PSPs were investigated,and the Box-Behnken experiment design was used to optimize the extraction of PSSs and PSPs.Results:The optimum extraction parameters of PSSs and PSPs were as follows:ultrasonic temperature was 50 ℃,ultrasonic time was 50 min,ultrasonic solid-liquid ratio was 1∶25(g/mL),enzymolysis time was 2 h,enzyme dosage was 3 053 U/g,solid-liquid ratio was 1∶25(g/mL),hot water extraction temperature was 80 ℃,and hot water extraction time was 1 h.Under the control of these conditions,the total yield of PSSs and PSPs was 12.22％and the 27.07％.The inhibitory activities of the extract and their complexes(PSSs-PSPs)onα-glucosidase and α-amylase were verified,and their complexes also have a good inhibitory effect on those enzymes.Conclusion:The process conditions for the efficient synergistic extraction of polygonatin and polysaccharide were obtained after optimization.Compared with a single component,the hypoglycemic activity of their complexes was improved.
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维普
