葛根蛋白酶解物大孔树脂吸附工艺优化及其体外抗氧化活性研究
Optimization of macroporous resin adsorption process of enzymatic hydrolysis of Pueraria protein and its antioxidant activity in vitro by response surface method
岳奕含 1严铭铭 1邵帅 1丁奕元 1江婷1
作者信息
- 1. 长春中医药大学,吉林长春 130117
- 折叠
摘要
目的:优化大孔树脂吸附葛根蛋白酶解物工艺及其抗氧化活性研究.方法:采用Box-Behnken响应面法确定大孔树脂吸附葛根蛋白酶解物最佳工艺.以维生素C为对照,测定最佳工艺下纯化前后葛根蛋白酶解物的抗氧化活性.结果:大孔树脂的最佳吸附—解吸工艺为上样液质量浓度10.0 mg/mL,洗脱液流速2.6 mL/min,洗脱液乙醇体积分数74％,纯化后葛根蛋白酶解物含量提升至37.19％.大孔树脂纯化后的葛根蛋白酶解物对DPPH自由基、ABTS+自由基、羟自由基的清除率均强于吸附前的.结论:大孔树脂纯化后的葛根蛋白酶解物抗氧化效果良好,可以作为一种潜在的蛋白多肽应用于食品中.
Abstract
Objective:This study aims to optimize the adsorption of enzymatic hydrolysis of Pueraria protein by macroporous resin and maximize the antioxidant properties in vitro.Methods:The Box-Behnken response surface method was used to determine the optimization of adsorption of enzymatic hydrolysis of Pueraria protein.Using VC as a control,the antioxidant activity of Kase was determined before and after purification under the optimal purification process.Results:The optimal adsorption-desorption process of macroporous resin is:the mass concentration of the sample solution was 10.0 mg/mL,the flow rate of the eluent was 2.6 mL/min,and the volume fraction of ethanol in the eluent was 74％.After adsorption,the content of enzymatic hydrolysis of Pueraria protein increased to 37.19％.The scavenging rate of DPPH,ABTS+,and hydroxyl radicals of Pueraria Mirifica protease after adsorption was stronger than before adsorption.Conclusion:This study shows that enzymatic hydrolysis of Pueraria protein after adsorption of macroporous resin has good antioxidant effect,which can be used as a potential protein polypeptide in food,providing data reference for further research of enzymatic hydrolysis of Pueraria protein.
关键词
葛根蛋白/酶解物/大孔树脂/抗氧化活性/吸附Key words
Pueraria protein/enzymatic hydrolysis/macroporous resins/antioxidant activity/adsorption引用本文复制引用
基金项目
吉林省科技发展计划项目(20210401111YY)
吉林省发改委产业技术研究与开发项目(2023C027-4)
吉林省教育厅人文社科研究项目(JJKH20211006SK)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据