Optimization of macroporous resin adsorption process of enzymatic hydrolysis of Pueraria protein and its antioxidant activity in vitro by response surface method
Objective:This study aims to optimize the adsorption of enzymatic hydrolysis of Pueraria protein by macroporous resin and maximize the antioxidant properties in vitro.Methods:The Box-Behnken response surface method was used to determine the optimization of adsorption of enzymatic hydrolysis of Pueraria protein.Using VC as a control,the antioxidant activity of Kase was determined before and after purification under the optimal purification process.Results:The optimal adsorption-desorption process of macroporous resin is:the mass concentration of the sample solution was 10.0 mg/mL,the flow rate of the eluent was 2.6 mL/min,and the volume fraction of ethanol in the eluent was 74％.After adsorption,the content of enzymatic hydrolysis of Pueraria protein increased to 37.19％.The scavenging rate of DPPH,ABTS+,and hydroxyl radicals of Pueraria Mirifica protease after adsorption was stronger than before adsorption.Conclusion:This study shows that enzymatic hydrolysis of Pueraria protein after adsorption of macroporous resin has good antioxidant effect,which can be used as a potential protein polypeptide in food,providing data reference for further research of enzymatic hydrolysis of Pueraria protein.
国家科技期刊平台
NSTL
万方数据
维普
