Migration quality of formaldehyde in the polylactic acid straw detected by HPLC
Objective:A formaldehyde detection method was established for the accurate analysis of the migration of formaldehyde in water and acid simulants from the polylactic acid straws.Methods:The preferred chromatographic conditions were:mobile phase water-acetonitrile(Vwater∶Vacetonitriie of 55∶45),column temperature of 35 ℃,detection wavelength of 355 nm.The optimized 2,4-dinitrophenylhydrazine derivative conditions were:the acetic acid in acidic 2,4-dinitrophenylhydrazine solution of 1.5 mL/100 mL(pH 3.07),the molar ratio of 2,4-dinitrophenylhydrazine of quadruple formaldehyde,the reaction temperature of 45 ℃,and the reaction time of 40 min.Results:The linear equation of formaldehyde-2,4-dinitrobenzene was y=55.247 0x-3.849 4 within 1.008 to 70.560 μg/mL,with the correlation coefficient of 0.999 5.The detection limit of the corresponding formaldehyde was 0.066 mg/kg,and the quantification limit of which was 0.22 mg/kg.The formaldehyde-DNPH was stable within 13.5 hours.The average recovery of simulants spiking with formaldehyde in 3 levels was from 92.6％to 104.1％,with SD(n=6)of 0.4％to 3.3％.The maximum migration of formaldehyde from disposable polylactic acid straws to three food simulants(water,3％acetic acid solution,4％acetic acid solution)was 1.839 mg/kg,less than the specific migration requirement of 15 mg/kg stipulated by China and the European Union.Conclusion:This method is accurate and efficient enough to apply for the analysis of formaldehyde migration from polylactic acid straws.
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