Objective:A high performance liquid chromatography method was developed to determine EDTA and NT A in water.Methods:The samples were derivatized with ferric chloride,used methanol and a 0.2 mmol/L tetrabutylammonium bromide phosphate buffer solution(pH 2.5)as the mobile phase,separated on a Thermo-C18(4.6 mm × 250 mm)column after gradient elution,detected by HPLC with a diode array detector,and used external standard method for quantitation.Results:The content of EDTA and NTA showed a good linear relationship in the range of 0.1～2.0 mg/L,the correlation coefficients were 0.999 5 and 0.999 2 respectively;The limits of detection were 0.05 mg/L and limits of quantification were 0.1 mg/L;The average recoveries were 93.5％～111.4％;And the relative standard deviations(RSD)were 3.63％～7.39％.Conclusion:This method was convenient,repeatable,sensitive,and practical for the detection of EDTA and NTA.
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