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苦荞酒酒糟中酚类化合物成分分析及其体外抗氧化活性

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目的:探究苦荞酒酒糟中酚类化合物的组成及含量,并研究其抗氧化活性。方法:通过高效液相色谱和液相质谱鉴定苦荞酒酒糟中酚类化合物的组成,并评价苦荞酒酒糟的体外抗氧化活性。结果:苦荞酒酒糟中共鉴定出7种酚类化合物,包括5种黄酮类化合物和2种酚酸类化合物,其中芦丁含量最高为(9。350±0。050)mg/g、其次为槲皮素、异槲皮素、山奈酚-3-o-芸香糖苷及山奈酚。当苦荞酒糟提取液质量浓度为0。5 mg/mL时,对羟自由基、ABTS+自由基、DPPH自由基的清除率分别为(73。29±0。09)%,(96。21±0。25)%,(82。55±0。68)%,FRAP值为(2。49±0。09)mmol/L。结论:发酵后的苦荞酒酒糟提取物具有一定的抗氧化活性,在低浓度条件下,对羟自由基和ABTS+自由基仍表现出较好的清除效果。
Phenolic compositions and in vitro antioxidant activities of tartary buckwheat wine lees
Objective:Explored the composition and content of phenolic compounds in tartary buckwheat wine lees,and study their antioxidant activity.Methods:Phenolic compounds in tartary buckwheat wine lees were analyzed by HPLC and LC-MS.And evaluating its antioxidant activity.Results:7 phenolic compounds were identified from tartary buckwheat wine lees,including 5 flavonoids,with the highest content of rutin of(9.350±0.050)mg/g,followed by quercetin,isoquercetin,kaempferol-3-o-rutoside and kaempferol of two phenolic compounds.At 0.5 mg/mL,the scavenging rates of hydroxyl free radical,ABTS+ and DPPH free radical reached(73.29± 0.09)%,(96.21±0.25)%and(82.55±0.68)%,respectively,with FRAP value of(2.49±0.09)mmol/L.Conclusion:The fermented tartary buckwheat lees are rich in phenolic compounds,and the lees extracts has certain antioxidant activity.In addition,at low concentration,the tartary buckwheat wine lees also showes good scavenging effects on hydroxyl radical and ABTS+ radical.

tartary buckwheat wineleesphenolic compositionsmaterial compositionantioxidant activity

赵佳伟、郑佳、李茂、张伟建、晏俊玲

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固态发酵资源利用四川省重点实验室,四川 宜宾 644000

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苦荞酒 酒糟 酚类化合物 物质组成 抗氧化活性

2024

食品与机械
长沙理工大学

食品与机械

CSTPCD北大核心
影响因子:0.89
ISSN:1003-5788
年,卷(期):2024.40(2)
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