Objective:This study aimed to optimize the microwave assisted aqueous two-phase extraction(MATPE)process of polysaccharides from Cornus officinalis and obtain a uniform polysaccharide fraction.Methods:The best extraction technology was determined through an orthogonal experiment.To obtain a uniform polysaccharides fraction(COP-2-S),the crude extract of C.officinalis polysaccharides was purified using DEAE-52 cellulose and Sephadex G-100 column chromatography.The molecular weight of COP-2-S was determined by high performance gel permeation chromatography.The monosaccharide composition of COP-2-S was determined by gas chromatography.The structure of COP-2-S was characterized by UV,and IR spectra,and a scanning electron microscope.Results:The optimal MATPE process of polysaccharides from C.officinalis was as follows:microwave power of 300 W,ethanol volume fraction of 35％,ammonium sulfate mass fraction of 22％,a solid-to-liquid ratio of 1∶20(g/mL),and the yield of polysaccharides from C.officinalis was(12.04±0.17)％.The molecular weight of COP-2-S was 17 450 Da.The molar ratio of arabinose,glucose,and galactose that made up the monosaccharide was 12.85∶30.71∶18.09.Conclusion:COP-2-S had no characteristic absorption at 260 nm and 280 nm.Besides,COP-2-S had typical infrared absorption characteristics of polysaccharides.
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