Objective;To achieve rapid,quantitative and high-throughput detection of aminoglycoside antibiotic residues in pork.Methods:Square-array aligned SERS substrates based on Au nanoflowers(AuNFs)were prepared using PP synthetic paper as a substrate.Pork samples were pre-treated by QuEChERs method and subjected to SRES.Results:Using 4-mercaptobenzoic acid as the SERS probe molecule,the substrate exhibited good homogeneity,SERS enhancement effect,reproducibility and stability.the SERS signal intensities at the characteristic peaks at 475,619 cm-1 showed good linear relationships with the logarithms of the concentrations of gentamicin sulphate and neomycin sulphate,respectively(R2 of 0.991 6 and 0.990 7,respectively).The limits of detections(LODs)were as low as 1×10-9,1×10-8 mol/L,respectively,and were successfully applied to the rapid,quantitative and high-throughput determination of aminoglycoside antibiotics in pork.Conclusion:The experimental method provides an economical,efficient,time-saving and highly sensitive way for the application of SERS technology for the detection of antibiotic residues in real meat samples.
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