Determination of 7 synthetic colorants in freshly prepared beverages by high performance liquid chromatography dual wavelength method with automatic solid phase extraction
Objective:A method for the determination of 7 synthetic colorants in freshly prepared beverages by high performance liquid chromatography dual(HPLC)wavelength method with automatic solid phase extraction was established.Methods:Extraction conditions:The sample was extracted 2 times with pure water,and then the supernatant combined after centrifugation.The pH of the supernatant was adjusted to 3～4.After activation by an automatic solid-phase extraction device,loading,rinsing,and elution of the sample were carried out on the Poly-sery synthetic colorant solid-phase extraction column.The eluent was concentrated to 200 μL by nitrogen blowing,and then fixed to 1.0 mL with 50％mobile phase A+50％mobile phase B.Instrument conditions:The column was C18 column.The mobile phase was 20 mmol/L ammonium acetate-methanol,gradient elution.The flow rate was 1.0 mL/min.The injection volume was 10 μL.The detection wavelength were 254 nm and 628 nm.Results:The correlation coefficient of each substance in the range of 0.5～50 μg/mL was greater than 0.999 9,the detection limit of the method was 0.023～0.179 mg/kg,the quantitative limit was 0.078～0.598 mg/kg,the standard recovery was 92.4％～96.8％,and the relative standard deviation(RSD)was 1.2％～4.2％.Conclusion:This method has low consumption of toxic reagents,simple operation steps,high degree of automation,good recovery and repeatability,and it is suitable for the determination of large quantities of samples.
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