Determination the residues of matrine and oxymatrine in Liupao tea by UPLC-MS/MS with isotope internal standard method
Objective:Taking Guangxi Liupao tea as an example,an UPLC-MS/MS analysis method with residual amounts of matrine and Oxymatrine was established.Methods:The prepared samples were subjected to optimized acetonitrile aqueous solution(80％acetonitrile+0.2％ammonia water)ultrasonic extraction,mobile phase:formic acid water and formic acid methanol(both 0.1％),gradient elution,and internal standard method quantification.Results:It was linear in the range of 0.1～80.0 ng/mL,r was greater than 0.999 6,the detection limits of matrine and oxymatrine were 1.0 μg/kg,and the quantitative limits were 3.0 μg/kg,which met the regulatory determination requirements for the residue of matrine and oxidative matrine in tea.Through the spike verification,the recovery rate was in the range of 92.0％～104.7％,and the relative standard deviation(RSD)was less than 5.4％.Conclusion:Method combined with purification tube purification(147.7 mg PSA,15.1 mg GCB,887.2 mg magnesium sulfate),using Kinetex® 2.6 μm Biphenyl 100 Å chromatographic column(100 mm X 3.0 mm)separation solves the problems of low extraction recovery rate and complex pre-treatment of matrine and oxymatrine,so that the symmetrical peak shape and strong anti-interference ability of matrine and oxymatrine.
万方数据
维普
