The antitumor activity of Brassica rapa L.acid polysaccharides in vivo
Objective:To explore the effects of Brassica rapa L.acid polysaccharide 1(BRAP-1)on H226 cell tumor-bearing mice.Methods:60 mice were divided into the model group(0.1 mL/10 g),positive cisplatin[3 mg/(kg·2 d)],BRAP-1 low dose[50 mg/(kg·d)],BRAP-1 medium dose[100 mg/(kg·d)]and BRAP-1 high dose[200 mg/(kg·d)],During the experiment,the tumor growth of mice was observed,and the tumor inhibition rate and organ index were calculated;The levels of interleukin-18(IL-18),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in serum were detected by enzyme-linked immunosorbent assay.The gene expressions of IL-18,IL-1β,RIP-1(receptor-interacting protein-1),RIP3 and MLKL(mixed-lineage kinase domain-like protein 3)were detected by Real-time Quantitative PCR(q-PCR)and Western blot(WB).Results:Compared with the model group,the low and middle dose of BRAP-1 groups had significant tumor inhibition effects(P＜0.05),and the positive group and the high dose of BRAP-1 group had significant tumor inhibition effects(P＜0.01).Compared with other groups in the model group,the serum levels of IL-1β in the positive group and the high dose of BRAP-1 group were significantly increased(P＜0.01),IL-18 and TNF-a were significantly decreased in the positive group(P＜0.05),and were significantly increased in each dose of BRAP-1 group(P＜0.01).The results of q-PCR and Western blot showed that compared with the model group,the relative expression of IL-1βmRNA decreased with the increase of BRAP-1 dose(P＜0.01),while the relative expression of IL-18,MLKL,RIP1,and RIP3 mRNA and protein increased(P＜0.01).Conclusion:BRAP-1 can inhibit the growth of lung squamous carcinoma H226 cells by regulating the necrotizing apoptosis-related proteins MLKL,RIP1,and RIP3 and regulating immune cytokine levels.
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维普
