摘要
目的:探究芜菁酸性多糖一组分(BRAP-1)对非小细胞肺癌H226细胞荷瘤小鼠的影响.方法:将60只小鼠分为模型组(0.1 mL/10 g)、阳性药物顺铂组[3 mg/(kg·2 d)]、BRAP-1 低剂量组[50 mg/(kg·d)]、BRAP-1 中剂量组[100 mg/(kg·d)]和 BRAP-1 高剂量组[200 mg/(kg·d)].试验期间观察小鼠肿瘤生长情况,计算抑瘤率、脏器指数;酶联免疫法检测各组小鼠血清中的白细胞介素18(IL-18)、IL-1β和肿瘤坏死因子α(TNF-α)含量;实时荧光定量核酸扩增法(q-PCR)、免疫印迹试验检测IL-18、IL-1β、受体相互作用蛋白1(RIP-1)、RIP3、混合谱系激酶结构域样蛋白(MLKL)基因表达情况.结果:与模型组相比较,BRAP-1低、中剂量组抑瘤效果明显(P＜0.05),阳性组和BRAP-1高剂量组抑瘤率效果显著(P＜0.01);模型组与其他各组相比较,阳性组和BRAP-1高剂量组小鼠血清中IL-1β含量显著增加(P＜0.01),IL-18、TNF-a在阳性组中明显降低(P＜0.05),BRAP-1各剂量组中显著增加(P＜0.01);q-PCR、免疫印迹试验结果显示,与模型组相比IL-1β随给药剂量增加 mRNA相对表达量减少(P＜0.01),而IL-18、MLKL、RIP1、RIP3的mRNA及蛋白相对表达量增加(P＜0.01).结论:BRAP-1可以通过调节免疫细胞因子水平,上调坏死性凋亡相关蛋白MLKL、RIP1、RIP3抑制.肺鳞癌H226细胞体内生长.
Abstract
Objective:To explore the effects of Brassica rapa L.acid polysaccharide 1(BRAP-1)on H226 cell tumor-bearing mice.Methods:60 mice were divided into the model group(0.1 mL/10 g),positive cisplatin[3 mg/(kg·2 d)],BRAP-1 low dose[50 mg/(kg·d)],BRAP-1 medium dose[100 mg/(kg·d)]and BRAP-1 high dose[200 mg/(kg·d)],During the experiment,the tumor growth of mice was observed,and the tumor inhibition rate and organ index were calculated;The levels of interleukin-18(IL-18),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in serum were detected by enzyme-linked immunosorbent assay.The gene expressions of IL-18,IL-1β,RIP-1(receptor-interacting protein-1),RIP3 and MLKL(mixed-lineage kinase domain-like protein 3)were detected by Real-time Quantitative PCR(q-PCR)and Western blot(WB).Results:Compared with the model group,the low and middle dose of BRAP-1 groups had significant tumor inhibition effects(P＜0.05),and the positive group and the high dose of BRAP-1 group had significant tumor inhibition effects(P＜0.01).Compared with other groups in the model group,the serum levels of IL-1β in the positive group and the high dose of BRAP-1 group were significantly increased(P＜0.01),IL-18 and TNF-a were significantly decreased in the positive group(P＜0.05),and were significantly increased in each dose of BRAP-1 group(P＜0.01).The results of q-PCR and Western blot showed that compared with the model group,the relative expression of IL-1βmRNA decreased with the increase of BRAP-1 dose(P＜0.01),while the relative expression of IL-18,MLKL,RIP1,and RIP3 mRNA and protein increased(P＜0.01).Conclusion:BRAP-1 can inhibit the growth of lung squamous carcinoma H226 cells by regulating the necrotizing apoptosis-related proteins MLKL,RIP1,and RIP3 and regulating immune cytokine levels.
基金项目
国家自然科学基金(81960765)
新疆维吾尔自治区科技重大专项(2022A03007-3)
新疆天然药物活性组分与释药技术重点实验室项目(XJDX1713)
维普
万方数据