摘要
目的:挑选黑豆药用与食用的适宜品种.方法:测定了 9种黑豆的总酚酸、总黄酮、缩合单宁、总花色苷、总多糖和总蛋白质含量,并对黑豆提取物的体外抗氧化活性以及对α-淀粉酶、胰脂肪酶、乙酰胆碱酯酶、酪氨酸酶的抑制作用进行比较.结果:总酚酸、总黄酮、缩合单宁、总花色苷含量为马料豆>小黑豆>黑豆;总多糖含量为小黑豆>黑豆>马料豆;总蛋白含量为马料豆>黑豆>小黑豆;抗氧化活性为马料豆>小黑豆>黑豆.体外试验发现,小黑豆与黑豆对多种酶均有抑制作用,强度各不相同.结论:不同品种黑豆的生物活性物质含量和功能存在差异.黑豆更适合食用,马料豆和小黑豆药用价值更高.
Abstract
Objective:The difference in bioactive substance content and bioactivity in black beans were compared.Methods:The contents of total phenolic acids,total flavonoids,condensed tannins,total anthocyanins,total polysaccharides and total proteins of nine kinds of black beans were determined.The antioxidant activities of black bean extracts in vitro and their inhibitory effects on a-amylase,pancreatic lipase,acetylcholinesterase and tyrosinase were compared.Results:The contents of total phenolic acid,total flavonoids,condensed tannins and total anthocyanins were as follows:equine bean>small black bean>black bean;Total polysaccharide content:small black bean>black bean>horse bean;Total protein content:horse bean>black bean>small black bean.Antioxidant activity:horse bean>small black bean>black bean.In vitro experiments showed that little black bean and black bean had inhibitory effects on many enzymes,and the intensity was different.Conclusion:The contents and functions of bioactive substances in different varieties of black beans are different.Black beans are more suitable for eating,and horse bean and small black beans have higher medicinal value.
基金项目
陕西省重点研发计划(2023-ZDLNY-37)
西安市科技局农业技术研发项目(21NYYF062)
西安医学院省级大学生创新创业训练计划(S202211840071)