Determination of six human milk oligosaccharides in dairy product by precolumn derivatization high performance liquid chromatography with fluorescence detection
Objective:To astablish a strong popularity,high sensitivity of six human milk oligosaccharides in dairy product by precolumn derivatization high performance liquid chromatography-fluorescence method.Methods:Aquatic product sample was precipitated with glacial acetic acid,filtration by using the filter paper.It was derived by 2-aminobenzamide solution.The extracts were centrifuged and filtered by using the organic filter membrane and analyzed by using a AdvanceBio Glycan Map column to be separated and detected,employing acetonitrile-50 mmol/L ammonium formate solution(pH 4.4)as the mobile phase for gradient elution.Results:Under the experimental conditions,six human milk oligosaccharides in dairy product were linear in the range of 1.00～400.0 mg/L,and the correlation coefficients were all over 0.999.The detection limit of the method was 4.1～10.9 mg/kg,the limits of quantification were 12.3～32.7 mg/kg,the mean recoveries rate was ranged from 71.3％to 90.2％with the intra-day precisions(n=6)in the range of 1.0％～6.3％and inter-day precisions(n=6)less than 10.0％.Conclusion:This method can meet the requirements of the daily detection of six human milk oligosaccharides due to its simplicity and sensitives.
万方数据
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