Determination of six human milk oligosaccharides in dairy product by precolumn derivatization high performance liquid chromatography with fluorescence detection
Determination of six human milk oligosaccharides in dairy product by precolumn derivatization high performance liquid chromatography with fluorescence detection
Objective:To astablish a strong popularity,high sensitivity of six human milk oligosaccharides in dairy product by precolumn derivatization high performance liquid chromatography-fluorescence method.Methods:Aquatic product sample was precipitated with glacial acetic acid,filtration by using the filter paper.It was derived by 2-aminobenzamide solution.The extracts were centrifuged and filtered by using the organic filter membrane and analyzed by using a AdvanceBio Glycan Map column to be separated and detected,employing acetonitrile-50 mmol/L ammonium formate solution(pH 4.4)as the mobile phase for gradient elution.Results:Under the experimental conditions,six human milk oligosaccharides in dairy product were linear in the range of 1.00～400.0 mg/L,and the correlation coefficients were all over 0.999.The detection limit of the method was 4.1～10.9 mg/kg,the limits of quantification were 12.3～32.7 mg/kg,the mean recoveries rate was ranged from 71.3％to 90.2％with the intra-day precisions(n=6)in the range of 1.0％～6.3％and inter-day precisions(n=6)less than 10.0％.Conclusion:This method can meet the requirements of the daily detection of six human milk oligosaccharides due to its simplicity and sensitives.
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