Optimization of preparation process and preliminary structure characterization of polysaccharides from lemon peel with high hypoglycemic activity
Objective:This study aimed to optimize the preparation process of polysaccharides from lemon peel and screen out the components with high hypoglycemic activity.Methods:Ultrasonic assisted enzymatic extraction of lemon peel polysaccharide,combined with response surface test to explore the best extraction process,through DEAE-52 anion chromatography and Sephadex G-100 gel column chromatography separation and purification to obtain a homogeneous polysaccharide fraction(named as LPs-1a).The effective hypoglycemic components of lemon peel polysaccharides were screened by α-glucosidase and their relative molecular weight,monosaccharide composition and characteristic groups were analyzed.Results:The optimal extraction process of lemon peel polysaccharide was ultrasonic treatment for at 55 ℃ for 44 min with solid-liquid ratio 1∶20(g/mL)and cellulase addition rate 1.1％.Under the control of these conditions,the yield of lemon peel polysaccharide was 6.83％,which was close to the predicted value.The LPs-1a fraction significantly inhibited the activity ofα-glucosidase with(1.22±0.04)mg/mL of IC50(P＜0.05).The relative molecular weight of this component was 195 260,and it was connected by α-glycosidic bonds and β-glycosidic bonds with a pyranose ring.The morphology of the polysaccharide was fibrous,and it was composed of mannose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose and arabinose Conclusion:Ultrasound-assisted enzymatic method is helpful in screening the highly hypoglycemic active components of lemon peel polysaccharides.
万方数据
维普
