Optimization of cellulase-assisted extraction of volatile oil from Acorus tatarinowii and study on its inhibitory components on GABA-T activity
Objective:This study aimed to establish a hollow fiber ligand fishing method for screening γ-Aminobutyrate transaminase(GABA-T)inhibitory component from the volatile oil of Acorus tatarinowii.Methods:Based on optimizing the enzyme assisted extraction process of volatile oil from A.tatarinowii using response surface methodology,GABA-T solution was injected into the hollow fiber lumen and used as a"bait"to fish out the potential active components in A.tatarinowii volatile oil,and its GABA-T inhibitory activity was verified.Results:The results showed that the optimum extraction conditions of volatile oil from A.tatarinowii were as follows:cellulase content 3 165 U/g,enzymolysis time 1.8 h,enzymolysis temperature 45 ℃,and the yield of volatile oil from A.tatarinowii was 2.56％.Finally,51 compounds were identified,mainly including olefins,alcohols,ethers,ketones,phenols,acids,and other compounds.3 mg/mL of GABA-T solution was injected into the cavity of the U-shaped hollow fiber tube,incubated for 160 min,sealed,and placed in the volatile oil of A.tatarinowii for ultrasonic(60 kHz)fishing for 20 min,and then analyzed with LCMS-IT-TOF system.Successfully filtered out α-Asarone has a concentration-dependent inhibitory effect on GABA-T activity,with an IC50 of 57.9 μg/mL.Conclusion:The established hollow fiber ligand fishing method is stable,feasible,and easy to operate.
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