Large-scale production of milk source CGMP by ion exchange resin method and its hemagglutination inhibitory activity against influenza virus
Objective:This study aimed to optimize the process conditions for the large-scale production of milk-derived casein glycomacropeptide(CGMP)and explore its hemagglutination inhibition(HI)ability against influenza virus.Methods:Using anion exchange resin as the separation medium and sialic acid content the activity detection index of the product,based on the preliminary small and medium-sized tests,the large-scale production of CGMP from whey powder was investigated from five process conditions:sample volume,loading flow,eluent concentration,eluent volume,and resin service cycle.Meanwhile,a hemagglutination inhibition test was used to verify the inhibitory activity of the product CGMP against the influenza virus.Results:The optimized technical route of CGMP product is as follows:loading volume 3BV,loading flow 0.8 L/min,elution volume 1BV,eluent concentration 0.6 mol/L,and suitable use cycle of resin 4 times.Under these conditions,the volume of whey powder solution was 108 L(2 160 g)and the resin was 36 L,and 21.183 g of CGMP product was obtained,with a yield of 0.981 g/100 g,the product purity of 89.032％,the recovery rate of 51.972％and the sialic acid content of 21.503 g/100 g.The minimum inhibitory concentration of CGMP products on influenza virus A(H1N1)and influenza virus B is 1.000 0 and 1.250 0 mg/mL,respectively.Conclusion:The purity and sialic acid content of CGMP produced by the optimized technical route were better than those of commercial CGMP,and had an obvious inhibitory effect on the influenza virus.
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