Colorimetric detection of 6-benzylaminopurine residues in vegetables based on Fe3O4 magnetic molecular-imprinted nanoparticles
[Objective]Achieving a rapid,sensitive,and visual detection of 6-benzylaminopurine(6-BA)residues in vegetables.[Methods]Based on the peroxidase-like activity and specific recognition of Fe3O4 magnetic molecularly imprinted nanoparticles(Fe3O4 MMIP NPs),a simple,rapid and highly selective colorimetric method was established to detect 6-BA residues in vegetables.[Results]Fe3O4 MMIP NPs exhibits optimal peroxidase-like catalytic activity at pH 4.0,0.02 mol/L TMB,0.02 mol/L H2O2 and a reaction time of 5 min.6-BA showed a good linear relationship with the difference between the blank and the sample absorbance value in the mass concentration range of 1～300 ng/mL with a limit of detection at 0.697 ng/mL.The method was successfully applied to the detection of 6-BA residues in vegetables(cucumber,tomato,bean sprouts,mung bean sprouts).The recovery rate was 83.47％～106.76％,with a relative standard deviation of 0.37％～5.66％.In addition,Fe3O4 MMIP NPs could still maintain good catalytic activity after 60 days of storage at 4 ℃.[Conclusion]The colorimetric method for the detection of 6-BA based on Fe3O4 MMIP NPs has the advantages of simplicity,rapidity,economy,and can be used for the detection of 6-BA residues in vegetables,which has certain reliability and practicability.
magnetic molecularly imprinted nanoparticlesperoxidase-like6-benzylaminopurinecolorimetric detectionvegetables
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