基于Fe3O4磁分子印迹纳米粒子比色检测蔬菜中6-苄氨基腺嘌呤残留
Colorimetric detection of 6-benzylaminopurine residues in vegetables based on Fe3O4 magnetic molecular-imprinted nanoparticles
刘艺丹 1栗鑫 1王婧丽 1李脉泉 1刘霞1
作者信息
- 1. 湖南农业大学食品科学技术学院,湖南长沙 410128;食品科学与生物技术湖南省重点实验室,湖南长沙 410128
- 折叠
摘要
[目的]实现蔬菜中6-苄氨基腺嘌呤(6-BA)残留的快速、灵敏、可视化检测.[方法]基于Fe3O4磁分子印迹纳米粒子(Fe3O4 MMIP NPs)的类过氧化物酶活性和特异识别性,构建了一种简单、快速、高选择性检测6-BA的比色方法,并用于蔬菜中6-BA残留检测.[结果]在pH 4.0、H2O2 浓度 0.02 mol/L、TMB 浓度 0.02 mol/L和反应时间5 min时,Fe3O4 MMIP NPs表现出最佳的类过氧化物酶催化活性.6-BA在1～300 ng/mL质量浓度范围内与空白和样品吸光度值的差值呈良好的线性关系,检测限为0.697 ng/mL.该方法被成功应用于蔬菜(黄瓜、西红柿、黄豆芽、绿豆芽)中6-BA残留检测,回收率为83.47％～106.76％,相对标准差为0.37％～5.66％.此外,4 ℃下,Fe3O4 MMIP NPs贮存60 d后仍能保持良好的催化活性.[结论]基于Fe3O4MMIP NPs检测6-BA的比色方法具有简便、快速、经济等优势,能够用于蔬菜中6-BA残留检测,具备一定的可靠性和实用性.
Abstract
[Objective]Achieving a rapid,sensitive,and visual detection of 6-benzylaminopurine(6-BA)residues in vegetables.[Methods]Based on the peroxidase-like activity and specific recognition of Fe3O4 magnetic molecularly imprinted nanoparticles(Fe3O4 MMIP NPs),a simple,rapid and highly selective colorimetric method was established to detect 6-BA residues in vegetables.[Results]Fe3O4 MMIP NPs exhibits optimal peroxidase-like catalytic activity at pH 4.0,0.02 mol/L TMB,0.02 mol/L H2O2 and a reaction time of 5 min.6-BA showed a good linear relationship with the difference between the blank and the sample absorbance value in the mass concentration range of 1～300 ng/mL with a limit of detection at 0.697 ng/mL.The method was successfully applied to the detection of 6-BA residues in vegetables(cucumber,tomato,bean sprouts,mung bean sprouts).The recovery rate was 83.47％～106.76％,with a relative standard deviation of 0.37％～5.66％.In addition,Fe3O4 MMIP NPs could still maintain good catalytic activity after 60 days of storage at 4 ℃.[Conclusion]The colorimetric method for the detection of 6-BA based on Fe3O4 MMIP NPs has the advantages of simplicity,rapidity,economy,and can be used for the detection of 6-BA residues in vegetables,which has certain reliability and practicability.
关键词
磁分子印迹纳米粒子/类过氧化物酶/6-苄氨基腺嘌呤/比色检测/蔬菜Key words
magnetic molecularly imprinted nanoparticles/peroxidase-like/6-benzylaminopurine/colorimetric detection/vegetables引用本文复制引用
基金项目
国家自然科学基金项目(31671931)
湖南省自然科学基金(2020JJ4354)
出版年
2024
国家科技期刊平台
NSTL
万方数据