The therapeutic effect of yam polysaccharide on dextran sodium sulfate-induced ulcerative colitis in mice
[Objective]This study aimed to investigate the effects of Yam Polysaccharide(YP)on ulcerative colitis(UC)mice.[Methods]The study included a blank group,YP group,DSS group,and DSS+YP group.The UC model was established by administering DSS water solution,and the treatment group received oral administration of YP for 7 days.Measurement of body weight,DAI score,inflammatory factors in the colon,NLRP3 inflammasome,and HE staining were conducted to evaluate the effects.RAW 264.7 macrophages were treated with 1 μg/mL lipopolysaccharide(LPS)for 24 h to establish the cell inflammation model in vitro.A certain concentration of YP solution was added to the culture medium and incubated with LPS for 24 h.The contents of IL-1β,IL-6 and TNF-α in the cell culture supernatance were detected by enzyme-linked immunosorbent assay.The expression level of NLRP3 inflammator-related protein was analyzed by Western blotting.[Results]Compared to the blank group,the DSS group showed weight loss,extensive ulceration in staining,increased DAI score,and elevated levels of inflammatory factors.Compared to the DSS group,the DSS+YP group showed significant improvement in intestinal damage,and the expression of IL-1β,IL-6,TNF-α,and activation of NLRP3 inflammasome signaling were significantly inhibited in intestinal tissues.In the cell inflammation model,YP pretreatment significantly suppressed the secretion of IL-1β,IL-6,TNF-α induced by LPS,as well as the expression of NLRP3 protein.[Conclusion]YP can improve DSS-induced UC in mice by inhibiting the activation of NLRP3 inflammasome.
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