暗褐脉柄牛肝菌发酵液多糖的分离纯化与结构表征
Isolation,purification and structure characterization of the polysaccharide from the fermentation broth of Phlebopus portentosus
潘章超 1王勇 1吴莉 1曾念开1
作者信息
- 1. 热带转化医学教育部重点实验室,海南海口 571199;海南省热带药用植物研究开发重点实验室,海南 海口 571199;海南医学院药学院,海南 海口 571199
- 折叠
摘要
[目的]高效利用暗褐脉柄牛肝菌资源,开发其多糖功能性食品.[方法]取暗褐脉柄牛肝菌菌丝发酵培养,低温减压浓缩,乙醇沉淀,DEAE-52纤维素柱色谱分离纯化,透析袋透析除去小分子杂质制备多糖.总糖含量采用苯酚—硫酸显色法测定,液质联用法测定单糖组成,紫外分光光度法判断是否存在核酸和蛋白等杂质;通过红外光谱和核磁共振波谱,对多糖的结构进行分析.[结果]制备的多糖总糖含量为84％,主要单糖种类有:葡萄糖、甘露糖、半乳糖和阿拉伯糖,其物质的量比为2.07∶1.95∶1.00∶1.58.红外光谱和核磁共振波谱分析具有多糖吸收峰,含α型吡喃糖.[结论]从暗褐脉柄牛肝菌发酵液中制备的多糖主要由4种单糖组成,结构中含α型吡喃糖.
Abstract
[Objective]This study aimed to efficiently utilize the resources of Phlebopus portentosus and develop its polysaccharide functional food.[Methods]The mycelium of P.portentosus was cultured and fermented,and the combined fermentation broth was concentrated at low a temperature and reduced pressure.Then,precipitated by ethanol,separated,purified by DEAE-52 cellulose column chromatography,and the polysaccharide was finally refined by dialysis to remove the small molecular impurities.The total sugar content was determined by phenol-sulfuric acid colorimetry,the monosaccharide composition was determined by liquid chromatography-mass spectrometry(LC-MS),and the impurities such as nucleic acid and protein were detected by ultraviolet spectrophotometer.The structure of polysaccharides was analyzed preliminarily by infrared spectroscopy(IR)and nuclear magnetic resonance spectroscopy(NMR).[Results]The content of total sugar was 84％.The main monosaccharides of the polysaccharide were glucose,mannose,galactose,and arabinose,the molar ratio of which was 2.07∶1.95∶1.00∶1.58.Obvious polysaccharide absorption peaks were shown in the infrared spectrum and nuclear magnetic resonance spectra,through which an α-type pyranose can be inferred in the polysaccharide.[Conclusion]The polysaccharides prepared from the fermentation broth of P.portentosus were mainly composed of four monosaccharides,the structure of which contained α-pyranose.
关键词
暗褐脉柄牛肝菌/发酵液/多糖/分离/纯化/结构表征Key words
Phlebopus portentosus/fermentation broth/polysaccharide/separation/purification/structural characterization引用本文复制引用
基金项目
海南省自然科学基金高层次人才项目(820RC623)
出版年
2024
国家科技期刊平台
NSTL
万方数据