顺序式模拟移动床色谱高效纯化低聚异麦芽糖应用研究
Application on efficient purification of isomaltooligosaccharides by sequential simulated moving bed chromatography
阮圣慧 1郑喜群 1刘晓兰 2李良玉 3姜彩霞3
作者信息
- 1. 黑龙江八一农垦大学食品学院,黑龙江大庆 163319;黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆 163319;粮食副产物加工与利用教育部工程研究中心,黑龙江大庆 163319
- 2. 齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔 161006
- 3. 黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆 163319;粮食副产物加工与利用教育部工程研究中心,黑龙江大庆 163319
- 折叠
摘要
[目的]提高低聚糖行业的生产效率.[方法]以50型低聚异麦芽糖浆为原料,利用顺式模拟移动床色谱分离技术对其纯化.通过制备色谱单柱试验筛选出钾离子型树脂为合适的分离介质;根据物料平衡原理和顺序式模拟移动床基本原理近似确定分离工艺的初始操作参数,并对分离条件进行优化.[结果]最佳技术参数为:料液折光率60％、柱温65 ℃、进样量455 mL、进水量682 mL、进样流量 37.4 mL/min、进水流量 29.6 mL/min以及总循环流量364 mL/h,在此条件下低聚异麦芽糖出口折光为20.40％,纯度达到90.20％,收率达到85.50％.[结论]自主研制的顺序式模拟移动床色谱分离设备可用于高效纯化低聚异麦芽糖.
Abstract
[Objective]To improve the production efficiency of the oligosaccharide industry.[Methods]Isomaltooligosaccharides 50 syrup was used as the raw material,and purified by sequential simulated moving bed chromatography.Potassium ion resin was selected as the suitable separation medium by preparative chromatography single column experiment.Then,based onthe principle of material balance and the basic principle of sequential simulated moving bed,the initial operating parameters of separation process were approximately established.Further the separation conditions were optimized.[Results]The best technical parameters were as follows:feed refraction was 60％and column temperature was 65 ℃.At the same time quantity of flow in the entrance and eluent were 455 mL/h and 682 mL/h,respectively.The velocity of flow in the entrance and eluent were 37.4 mL/min and 29.6 mL/min,respectively.Besides,the circulating flow was kept within 364 mL/h.Finally,the outlet refraction of isomaltooligosaccharides was 20.40％with the purity of 90.20％and the yield of 85.50％.[Conclusion]The sequential simulated moving bed chromatographic separation equipment independently developed by the laboratory can efficiently purify isomaltooligosaccharides.
关键词
顺序式模拟移动床/色谱分离/纯化/低聚异麦芽糖Key words
sequential simulated moving bed/chromatographic separation/purification/isomaltooligosaccharides引用本文复制引用
基金项目
黑龙江省"百千万"工程科技重大专项(2019ZX06B02-1)
黑龙江八一农垦大学研究生创新科研项目(YJSCX2022-Y47)
出版年
2024
国家科技期刊平台
NSTL
万方数据