摘要
[目的]提高藜麦种子有机硒含量,开发优质富硒藜麦食品原料.[方法]以青白藜1号为原料,亚硒酸钠为硒源,通过单因素试验结合正交试验对藜麦富硒萌发工艺条件进行优化,进一步对富硒藜麦可溶性蛋白、多酚含量及抗氧化能力进行分析.[结果]藜麦富硒萌发最佳工艺条件为亚硒酸钠溶液质量浓度20 mg/L、浸泡温度25 ℃、浸泡时间6 h、浸泡液pH 7.0、萌发时间48 h、萌发温度20 ℃.该条件下,富硒萌发藜麦有机硒含量为349.52 μg/kg.富硒萌发藜麦游离酚(236.35 mg/100 g)、结合酚(198.31 mg/100 g)含量最高,普通去离子水萌发藜麦可溶性蛋白(22.18％)含量最高.富硒萌发藜麦可溶性蛋白、游离酚和结合酚的抗氧化活性均最强,其次为普通去离子水萌发藜麦,未处理藜麦相对最低.[结论]富硒萌发能够显著提升藜麦有机硒、可溶性蛋白和多酚含量,并增强其抗氧化能力.
Abstract
[Objective]This study aimed to improve the organic selenium content of quinoa seeds and develop high-quality Se-rich quinoa food raw materials.[Methods]Using Qingbaili No.1 quinoa as raw material and sodium selenite as a selenium source,the selenium-enriched germination process conditions of quinoa were optimized through one-way experiments combined with orthogonal experiments.The soluble protein,polyphenol content,and antioxidant capacity of selenium-enriched quinoa were further analyzed.[Results]The optimal process conditions for selenium-enriched germination of quinoa were sodium selenite solution concentration of 20 mg/L,soaking temperature of 25 ℃,soaking time of 6 h,soaking solution pH of 7.0,germination time of 48 h,and germination temperature of 20 ℃.Under these conditions,the organic selenium content of selenium-enriched germinated quinoa was 349.52 μg/kg.Selenium-enriched sprouted quinoa had the highest content of free phenols(236.35 mg/100 g)and bound phenols(198.31 mg/100 g),and control deionized water germinated quinoa had the highest content of soluble protein(22.18％).Notably,the antioxidant activities of soluble protein,free phenols,and bound phenols in selenium-rich germinated quinoa were the strongest,followed by those in control deionized water germinated quinoa,and untreated quinoa was relatively the lowest.[Conclusion]Se-enriched germination can significantly increase the contents of organic selenium,soluble protein and polyphenols,and enhance the antioxidant capacity of Quinoa.
基金项目
青海"昆仑英才·高端创新创业人才"计划特色人才项目(2022-027)
国家科技期刊平台
NSTL
万方数据