Extraction of flavonoids from Goji(Lycium barbarum L.)leaves by ultrasonic-assisted two-phase aqueous method and the inhibitory activity of the extract on pancreatic lipase
[Objective]This study aimed to achieve high yield and green extraction of flavonoids from Goji(Lycium barbarum L.)leaves using ultrasonic assisted aqueous two-phase method,and to evaluate the activity of extract.[Methods]Single factor and PB(Plackett-Burman)experiments were conducted to investigate the influence of various factors on the extraction efficiency of flavonoids.Three parameters were chosen to optimize the extraction conditions using a Central Composite Design(CCD)of the response surface experiment:the amount of polyethylene glycol(PEG400),the amount of ammonium sulfate,and the solid-liquid ratio.The extract's activity was assessed using an activity inhibition test of pancreatic lipase.[Results]The optimal process conditions for ultrasonic-assisted aqueous two-phase extraction of flavonoids from Goji berry leaves were:28%polyethylene glycol 400,16%ammonium sulfate,ultrasonic power of 250 W,ultrasonic treatment time of 40 minutes,ultrasonic treatment temperature of 40℃,and solid-solid ratio of 1∶30(g/g).Under these conditions,the total flavonoid content of the extract was(486.30±3.29)mg/g,which had a significant inhibitory effect on pancreatic lipase activity,and the half inhibition concentration(IC50)was 1.06 mg/mL.[Conclusion]Under the optimal extraction conditions,the flavonoids extracted from L.barbarum leaves by ultrasonic-assisted aqueous phase method had high yield and low solvent residue,and the flavonoids from L.barbarum leaves obtained by this method had a good inhibitory effect on pancreatic lipase.
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