Determination of chlorothalonil and its metabolite 4-hydroxychlorothalonil residues in animal-derived food by ultra-high performance liquid chromatography-tandem mass spectrometry
Determination of chlorothalonil and its metabolite 4-hydroxychlorothalonil residues in animal-derived food by ultra-high performance liquid chromatography-tandem mass spectrometry
[Objective]To establish a method for simultaneous determination of chlorothalonil and its metabolite 4-hydroxychlorothalonil residues in animal-derived food by ultra-high performance liquid chromatography-tandem mass spectrometry.[Methods]The samples were extracted by oscillating with acetonitrile solution of 1%acetic acid,defatted with n-hexane,purified by matrix dispersible solid phase extractant(PSA,anhydrous magnesium sulfate),separated by Waters BEH C18 column under a gradient elution program using 0.1%formic acid aqueous solution and acetonitrile as the mobile phases.The anion multiple reaction monitoring model(MRM)of atmospheric pressure chemical ionization source(APCI)was used for the determination,and the matrix matching external standard method was used for quantitative determination.[Results]The linear relationships of chlorothalonil and 4-hydroxychlorothalonil were good in the range of 5～500 μg/L,and the limits of quantitation were 0.005 mg/kg.The average recoveries of chlorothalonil and 4-hydroxychlorothalonil were 69.3%～111.0%and 65.3%～117.9%,respectively.The relative standard deviations of chlorothalonil and 4-hydroxychlorothalonil were 1.6%～10.8%and 1.1%～9.0%,respectively.[Conclusion]The method is simple,rapid,sensitive,accurate and reliable for simultaneous determination of chlorothalonil and its metabolite 4-hydroxychlorothalonil in animal-derived food.
关键词
超高效液相色谱—串联质谱/百菌清/4-羟基百菌清/动物源性食品
Key words
ultra-high performance liquid chromatography-tandem mass spectrometry/chlorothalonil/4-hydroxychlorothalonil/animal-derived food
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