Optimization of the preparation process of compound Panax ginseng cream and its in vitro whitening effects
[Objective]This study aimed to optimize the production process of composite red ginseng cream to enhance its whitening effects.[Methods]The preparation process conditions of the composite Panax ginseng cream were optimized using orthogonal tests,assessing active ingredient content,antioxidant capacity,tyrosinase inhibition,and suppression of melanin production in B16 mouse melanoma cells.[Results]The optimal conditions for preparing the composite red ginseng cream were material-liquid ratio 1∶6(g/mL),inoculation of lactic acid bacteria 1%,and fermentation at 37℃for 2 d.Under the control of these conditions,the cream contained 15.5 mg/g of total saponins,8.2 mg/g of total flavonoids,and 331.5 mg/g of polysaccharides.The IC50 values for DPPH free radicals,ABTS free radicals,and PTIO free radicals were 561.5,423.1,and 512.1 μg/mL,respectively.While the IC50 values for inhibiting tyrosinase activity and melanin production in B16 mouse melanoma cells were 721.2 and 497.2 μg/mL,respectively.[Conclusion]The optimized preparation of red ginseng cream,using a material-liquid ratio of 1∶6(g/mL),1%lactic acid bacteria,and 2 d fermentation at 37℃,achieved effective whitening properties through antioxidant and melanin-inhibitory actions.
万方数据
维普
