基于ISO19036:2019评估饮用水中大肠菌群的测量不确定度
Estimation of measurement uncertainty of coliforms in drinking water according to ISO19036:2019
钟文涛 1卢琳 1李政 2周培华 1洪振柏 1周丛1
作者信息
- 1. 湖南省产商品质量检验研究院,湖南 长沙 410007;食品安全监测与预警湖南省重点实验室,湖南 长沙 410007
- 2. 湖南省产商品质量检验研究院,湖南 长沙 410007
- 折叠
摘要
[目的]参考ISO19036:2019对饮用水中大肠菌群的测量不确定度进行评估.[方法]通过设计试验方案,分别评估技术不确定度(utech)、基质不确定度(umatrix)和分布不确定度(udistrib),并综合3个分量的数值计算合成不确定度(uc(y))和扩展不确定度(U).[结果]对GB 4789.3-2016第二法进行测量不确定度评估后,得到的技术不确定度为0.120 2 log(CFU/mL),基质不确定度为0.1 log(CFU/mL),分布不确定度分别为0.065 5,0.026 1 log(CFU/mL),当结果为440 CFU/mL时,扩展不确定度为0.34 log(CFU/mL),表示为(2.64±0.34)log(CFU/mL).[结论]ISO19036:2019对食品中微生物定量检测方法进行测量不确定度评估相较目前国内标准更科学、更便捷.
Abstract
[Objective]This study aimed to estimate the measurement uncertainty of coliforms in drinking water according to ISO19036:2019.[Methods]By designing protocols,the technical uncertainty(utech),matrix uncertainty(umatrix)and distribution uncertainty(udistrib)were estimated respectively,and the combined uncertainty(uc(y))and extended uncertainty(U)were calculated by combining the values of the three components.[Results]After estimating the measurement uncertainty of the second method of GB 4789.3-2016,the technical uncertainty was 0.120 2 log(CFU/mL),the matrix uncertainty was 0.1 log(CFU/mL),and the distribution uncertainty was 0.065 5 log(CFU/mL)and 0.026 1 log(CFU/mL).When the result was 440 CFU/mL,the extended uncertainty was 0.34 log(CFU/mL),expressed as(2.64±0.34)log(CFU/mL).[Conclusion]According to ISO19036:2019,the estimation of measurement uncertainty for quantitative determinations of microorganisms in food is more scientific and convenient compared to current domestic standards.
关键词
测量不确定度/技术不确定度/基质不确定度/分布不确定度Key words
measurement uncertainty/technical uncertainty/matrix uncertainty/distributional uncertainty引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据