Optimization of the extraction process of ACE inhibitory peptides from distillery waste and their antioxidant activity
[Objective]This study aimed to handle distillery waste effectively,research and develop high-value components within distillery waste,and explore its potential application approaches.[Methods]Proteins were extracted from distillery waste throuth the ultrasonic-assisted alkali extraction and acid precipitation method.Angiotensin-converting enzyme(ACE)inhibitory peptides were prepared by enzymatic hydrolysis of the crude protein of distillery waste using a compound enzyme.The extraction process conditions of ACE inhibitory peptides were optimized by single factor experiments and response surface experiments,and their antioxidant activity was investigated.[Results]The optimal conditions for preparing ACE-inhibitory peptided by compound enzymatic hydrolysis were as follows:the ratio of enzyme(Vpapainase∶Vneutral protease)was 3∶1,and the hydrolysis was contacted at 55.41℃and pH 7.72,for 2.38 h.Under the control of these conditions,the hydrolysis degree of ACE-inhibitory peptide was 66.62%;the highest ACE inhibitory activity(79.05%)was obtained after 3 h of enzymatic hydrolysis.The scavenging rates of the purified and extracted ACE inhibitory peptides against DPPH and ABTS free radicals were 2.078%and 3.226%,respectively,and the total antioxidant capacities were 1.161 μmol/mL and 1.255 mmol.[Conclusion]The process of extracting ACE inhibitory peptide from distillery waste using the compound enzymatic hydrolysis method is feasible.
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