Optimization of extraction process of hemp seed protein by reverse micelle method and study on its physicochemical properties and digestibility
[Objective]The process was optimized and the physicochemical properties of the obtained hemp seed protein,extracted by using reverse micelle method,were analyzed.[Methods]The key factors affecting the extraction rate of protein,such as Aerosol-OT(AOT)mass concentration,water addition,buffer pH,KCl solution concentration,solid-liquid ratio,extraction temperature and time of pre-extraction process,and pH,KCl concentration,extraction time and temperature of post-extraction progress,were analyzed by single factor analysis and response surface optimization.The solubility,water holding capacity,oil holding capacity and digestion characteristics of the obtained protein were investigated.[Results]The optimal process conditions for the reverse micelle pre-extraction were obtained as follows:AOT mass concentration 0.090 4 g/mL,buffer pH 6.54,water addition 0.105 8 mL/mL,KCl concentration 0.05 mol/L,solid-liquid ratio 1∶30(g/mL),extraced for 60 min at 50℃.The pre-extraction rate was 71.38%.The optimal process conditions for the reverse micelle post-extraction were obtained as follows:pH 7.5,KCl concentration 1.5 mol/L,post-extraction time 45 min,temperature 27℃.The post-extraction rate was 80.59%,and the total extraction rate was 57.53%.The white and fluffy protein powder with purity of 98.12%was obtained by dialysis and freeze-drying.Its solubility changed significantly with pH values.The water holding capacity was 0.71 g/g,the oil holding capacity was 3.57 g/g,and the digestibility was 93.83%.[Conclusion]Reverse micelle method can be used to extract hemp seed protein.The characteristics of excellent oil holding capacity and high digestibility make hemp seed protein have unique advantages in food application.
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