3-氯-1,2-丙二醇通过线粒体途径诱导HK2细胞凋亡
3-Monochloropropane-1,2-diol induced cell apoptosis in HK2 cells via mitochondria-dependent pathway
晋程妮 1李洋 2闫帅帅 2徐建国 2彭晓丽3
作者信息
- 1. 山西师范大学食品科学学院,山西 太原 030000;西北农林科技大学食品科学与工程学院,陕西 咸阳 712100
- 2. 山西师范大学食品科学学院,山西 太原 030000
- 3. 西北农林科技大学食品科学与工程学院,陕西 咸阳 712100
- 折叠
摘要
[目的]探究食品污染物3-氯-1,2-丙二醇(3-monochloropropane-1,2-diol,3-MCPD)对人肾小管上皮细胞系(HK2)的毒性作用及机制.[方法]通过MTT法、Hoechst 33342染色法、试剂盒及Western blot检测了细胞存活率、形态、线粒体功能、酶活性及凋亡相关蛋白的表达.[结果]浓度低于80 mmol/L的3-MCPD处理24 h后,细胞存活率呈浓度依赖性降低.20,30 mmol/L的 3-MCPD处理不同时间后,细胞存活率呈时间依赖性降低.20,40,60 mmol/L的 3-MCPD引起LDH的释放率增加,线粒体膜电位和ATP水平降低,荧光显微镜观察可见高强度的蓝色荧光,核皱缩,凋亡小体等细胞凋亡现象.同时,3-MCPD处理能降低抗凋亡蛋白Bcl2的表达,增加促凋亡蛋白Bax的表达,提高Caspase 9和Caspase 3的酶活性,且呈浓度依赖性.[结论]3-MCPD对HK2细胞具有毒性作用,并通过线粒体途径诱导其凋亡.
Abstract
[Objective]This study investigated the toxic effects of the food contaminant 3-monochloropropane-1,2-diol(3-MCPD)on the human renal tubular epithelial cell line(HK2)and examined the underlying mechanisms.[Methods]Cell viability,morphology,mitochondrial function,enzyme activities,and apoptosis-associated proteins expressions were assessed using MTT assay,Hoechst 33342 staining,biochemical test kits and Western blot,respectively.[Results]Results showed a concentration-dependent decrease in cell viability when 3-MCPD was below 80 mmol/L for 24 h.Treatment with 20 and 30 mmol/L 3-MCPD over varying durations also led to a time-dependent reduction in cell viability.Additionally,3-MCPD at 20,40 and 60 mmol/L significantly increased LDH release,decrease mitochondrial membrane potential,and lowered ATP levels.Fluorescence microscopy revealed notable morphological changes,including bright blue fluorescence,nuclear shrinkage and apoptosis bodies.Furthermore,3-MCPD treatment downregulated the anti-apoptotic protein Bcl2,upregulated the pro-apoptotic protein Bax,and increased Caspase 9 and Caspase 3 activity in a dose-dependent manner.[Conclusion]3-MCPD exhibits toxic effects on HK2 cells and induces apoptosis through a mitochondria-dependent pathway.
关键词
3-氯-1,2-丙二醇/HK2细胞/线粒体/细胞凋亡Key words
3-monochloropropane-1,2-diol(3-MCPD)/HK2 cells/mitochondria/apoptosis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据