基于普鲁士蓝纳米花光催化降解黄曲霉毒素B1的研究
Photocatalytic degradation of aflatoxin B1 based on prussian blue nanoflower
张甜钰 1谢新辉 1吕艳 1鲁迨 2石星波1
作者信息
- 1. 湖南农业大学食品科学技术学院,湖南 长沙 410128;食品科学与生物技术湖南省重点实验室,湖南 长沙 410128
- 2. 湖南农业大学食品科学技术学院,湖南 长沙 410128;食品科学与生物技术湖南省重点实验室,湖南 长沙 410128;湖南中医药大学药学院,湖南 长沙 410208;中医药民族医药国际联合实验室,湖南 长沙 410208
- 折叠
摘要
[目的]实现黄曲霉毒素B1(AFB1)的高效、绿色降解.[方法]利用核酸介导法制备具有高效光芬顿性能的普鲁士蓝纳米花(PBNFs),探究其对AFB1的光降解性能及机理.[结果]在近红外光(NIR)照射下,PBNFs表现出优异光芬顿性能,4 h内可完全去除0.5 μg/mL的AFB1.经优化试验,过氧化氢(H2O2)浓度、PBNFs浓度和光照强度增加对光催化降解AFB1均有促进作用.PBNFs经NIR照射触发H2O2分解产生的活性氧破坏了AFB1的末端呋喃双键和苯环侧链.利用质谱分析AFB1的降解产物,并通过细胞毒性试验证明了AFB1降解产物的毒性明显降低.将该方法用于3种葡萄酒样品中AFB1的降解,降解率分别为66.6%,90.8%,63.4%.[结论]NIR驱动的PBNFs光催化降解AFB1的效率较高且具有绿色、无二次污染的优势.
Abstract
[Objective]To achieve efficient and environmentally friendly degradation of Aflatoxin B1(AFB1).[Methods]This work employs a nucleic acid-mediated methodology to synthesize Prussian Blue Nanoflowers(PBNFs)characterized by highly efficient photo-Fenton catalytic activity,investigating their photodegradation efficacy and mechanisms against Aflatoxin B1(AFB1).[Results]The findings reveal that,under near-infrared light irradiation(NIR),0.5 μg/mL of AFB1 can be completely eliminated within 4 h,which is attributed to the exceptional photo-Fenton performance of the PBNFs photocatalyst.Optimization experiments indicate that increasing the concentrations of hydrogen peroxide(H2O2)and PBNFs,along with enhancing light intensity,significantly facilitates the photocatalytic degradation of AFB1.Reactive oxygen species generated from H2O2 decomposition,triggered by NIR irradiation of PBNFs,effectively disrupt the terminal furan double bond and the benzene ring side chain of AFB1.Mass spectrometry analysis was conducted to elucidate the degradation products of AFB1,and cytotoxicity assays demonstrated a significant reduction in the toxicity of the AFB1 degradation products.The proposed method was applied to the degradation of AFB1 in three wine samples,achieving degradation rates of 66.6%,90.8%,and 63.4%,respectively.[Conclusion]This method provides an efficient and environmentally benign approach to the degradation of AFB1 in food samples.
关键词
普鲁士蓝纳米花/黄曲霉毒素B1/近红外光/过氧化氢/降解Key words
prussian blue nanoflowers/aflatoxin B1/near infrared light/hydrogen peroxide/degradation引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据