一株产细菌素乳酸菌的筛选鉴定及对金黄色葡萄球菌的抑菌作用
Screening and identification of a bacteriocin-producing lactic acid bacterium and its antibacterial effect on Staphylococcus aureus
杨洋洋 1徐云凤 1郭卫平 1罗磊 1向进乐1
作者信息
- 1. 河南科技大学食品与生物工程学院,河南 洛阳 471023
- 折叠
摘要
[目的]从泡菜中分离筛选出产细菌素的乳酸菌,对菌株进行鉴定并研究其对金黄色葡萄球菌的抑菌作用.[方法]以金黄色葡萄球菌为指示菌,将分离得到的目标菌株进行抑菌初筛后,通过酸排除、过氧化氢排除以及酶验证试验确定产细菌素菌株;对菌株进行生理生化鉴定和16S rDNA序列分析;通过最小抑菌质量浓度(MIC)测定、生长曲线测定、生物膜染色、扫描电子显微镜观察,探究细菌素粗提物的抑菌特性.[结果]筛选得到一株产细菌素菌株N-11,鉴定为植物乳杆菌;细菌素粗提物对金黄色葡萄球菌的MIC为(17.50±0.25)mg/mL,该粗提物能够明显抑制金黄色葡萄球菌的生长,对金黄色葡萄球菌的生物膜形成具有抑制效果,金黄色葡萄球菌经处理后细胞形态遭到不同程度破坏.[结论]试验筛选得到的植物乳杆菌所产细菌素粗提物对金黄色葡萄球菌抑菌作用良好,具有较高的开发利用价值.
Abstract
[Objective]This study aimed to isolate and screen bacteriocin-producing lactic acid bacteria from kimchi,identify the strains and investigate their antibacterial effects against Staphylococcus aureus.[Methods]S.aureus was employed as the indicator bacterium.Bacteriocin-producing strains were identified through acid elimination,hydrogen peroxide exclusion,and enzyme verification test.Physiological and biochemical identification,along with 16S rDNA sequencing,was conducted on the strains.The antibacterial properties of the bacteriocin extract were assessed using minimum inhibitory concentration(MIC)determination,growth curve analysis,biofilm staining,and scanning electron microscopy observations.[Results]A bacteriocin-producing strain,designated N-11,was successfully isolated and identified as Lactobacillus plantarum.The MIC of the crude bacteriocin extract against S.aureus was found to be(17.50±0.25)mg/mL,demonstrating a significantly ability to inhibit both the growth and the biofilm formation of S.aureus.[Conclusion]The crude extract producted by L.plantarum N-11 exhibits strong antibacterial effects against S.aureus,indicating its potential for further development and utilization in food preservation and safety applications.
关键词
乳酸菌/分离/鉴定/细菌素/抑菌作用/泡菜Key words
lactic acid bacteria/separation/identification/bacteriocin/antibacterial activity/pickle引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据