茶树菇降压肽制备工艺优化
Optimization of the preparation process of hypotensive peptide of Agrocybe cylindracea
伍津瑶 1殷明月 1杨美花 1康晶晶1
作者信息
- 1. 贵州医科大学公共卫生与健康学院,贵州 贵阳 550025;贵州医科大学环境污染与疾病监控教育部重点实验室,贵州 贵阳 550025
- 折叠
摘要
[目的]确定最优的茶树菇降压肽提取工艺,以期得到得率更高、活性更好的降压肽.[方法]以茶树菇为原料,以多肽得率与ACE抑制率为评价指标,探讨酶种类、底物浓度、酶浓度、pH、反应时间、反应温度等对茶树菇降压肽及活性的影响,并采用响应面法优化茶树菇降压肽的最佳提取工艺.[结果]茶树菇降压肽的最优提取条件为底物浓度5.4%,酶解时间8 h,酶解温度23.3℃,pH 8.0,中性蛋白酶添加量2 g,此时茶树菇降压肽得率为92.12%,ACE抑制率为93.75%.茶树菇降压肽活性成分主要分布于相对分子质量<3 kDa的组分中,该成分占总降压肽的36.69%,ACE抑制率为95.36%.[结论]试验优化了茶树菇降压肽的提取工艺,确定了茶树菇降压肽的相对分子质量组成.
Abstract
[Objective]This study aimed to determine the optimal extraction process for antihypertensive peptide from Agrocybe cylindracea to achieve higher yield and enhanced activity.[Methods]With the polypeptide yield and ACE inhibition rate as the evaluation index,the effects of enzyme type,substrate concentration,enzyme concentration,pH value,reaction time,reaction temperature and other single factors on the antihypertensive peptide and its activity were investigated,and the optimal extraction process of antihypertensive peptide was optimized by response surface.[Results]The optimal extraction conditions were as follows:substrate concentration of 5.4%,enzymolysis duration of 8 h,reaction temperature was 23.3℃,pH 8.0,and neutral protease dosage of 2 g.Under the control of these conditions,the antihypertensive peptide yield reached 92.12%,with an ACE inhibition rate of 93.75%.The active peptide fraction,primarily composed of peptides with molecular weight<3 kDa,accounted for 36.69%of the total peptide and demonstrated an ACE inhibition rate of 95.36%.[Conclusion]The extraction process for antihypertensive peptides from A.cylindracea was successfully optimized,and the molecular weight distribution of the active peptide components was determined.
关键词
茶树菇降压肽/酶法制备/多肽/工艺优化Key words
Agrocybe cylindracea antipressure peptide/enzymatic preparation/polypeptide/process optimization引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据