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高效酒精酵母菌的选育及鉴定

Screening and Identification of High Efficiency Alcohol Yeast

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从各种腐败水果、葡萄园里土壤等不同基质中筛选分离得到144株酵母菌,采用耐热、耐酒精处理方法,结合TTC法初筛和复筛试验得到一株酒精发酵的高产酵母菌株,编号为TJ-18。研究结果表明,该菌糖利用率较高,且发酵力突出,40℃培养72 h,CO2失重为10.4 g,酒精产率最高可达5.9%(体积分数)。对其形态及特征做了常规鉴定实验,根据生理生化试验结果,并对照《TheYeast》对酵母的描述,确定TJ-18为酵母属的酿酒酵母(Saccharomyces cerevisiae)。
From all kinds of corruption fruit, in the vineyard to soil and so on the different matrix screening separation have 144 strains of yeast, use heat resistant, resistant to alcohol processing methods, combined with TTC method initial screen and complex screen test got a strain of alcoholic fermentation yield yeast strain. Numbers for TJ-18. The results show that the bacteria sugar utilization rate is higher, And fermenting power is outstanding, 40℃culture 72 h, CO2 weightlessness is 10.4 g, alcohol yield the highest can amount to 5.9%(v/v). The form and characteristics of the conventional do identification experiment, according to the physiological and biochemical test results, and control the TheYeast"on the description of the yeast, determine the TJ-18 for Saccharomyces wine yeast (Saccharomyces cerevisiae).

themotolerant yeastscreeningappraisalalcoholic fermentation

李忠英、陈杰山、罗跃中、吴永尧

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湖南化工职业技术学院,湖南株洲 412004

湖南农业大学生物科学技术学院,湖南长沙 410128

耐高温酵母 筛选 鉴定 酒精发酵

湖南省教育厅科学研究项目湖南化工职业技术学院院级课题

12C1048HNHY2013001

2014

食品研究与开发
天津市食品研究所,天津市食品工业生产力促进中心

食品研究与开发

CSTPCD北大核心
影响因子:0.561
ISSN:1005-6521
年,卷(期):2014.(7)
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