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金银花叶抑菌成分的提取及效果研究

Study on Extraction and Activity of Effective Antibacterial Ingredients from Lonicera Japonica Leaves

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研究金银花叶抑菌成分的最佳提取工艺和抑菌效果。以抑菌圈大小为评价指标，通过比较普通回流、超声提取、索氏提取三种方法的效果确定相对较佳提取方法，并在单因素的基础上采用正交试验优化提取工艺，采用梯度稀释法测定提取物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。结果表明，最佳提取工艺为采用普通回流提取，温度为70℃、乙醇体积分数为60%、料液比为1∶15 g/mL的条件下提取时间1.5 h，金银花叶乙醇提取物对大肠杆菌和金黄色葡萄球菌的MIC分别为500μg/g和250μg/g，MBC分别为1000μg/g和500μg/g。结论：采用普通回流提取法能较好地提取金银花叶抑菌活性成分，工艺稳定、可行。
To study the optimum extraction process and activity of antibacterial ingredients from Lonicera Japonica Leaves. The inhibition zone as evaluation index, and the normal reflux, ultrasonic extraction and Soxhlet extraction had been compared to obtain the relatively effective extraction method , meanwhile orthogonal experiment had been investigated based on the single factor experiment. In addition , gradient dilution method have been used for researching MIC and MBC. The results showed that , the reflux method was selected at last and the optimum condition of antibacterial ingredients extraction was 60%ethanol in 1.5 h at 70℃and solid to liquid ratio 1 ∶ 15 g/mL. MIC was 250 μg/g and 500 μg/g for Staphylococcus aureus and Escherichia coli, respectively. MBC was 500μg/g and 1 000μg/g for Staphylococcus aureus and Escherichia coli, respectively. Conclusion, antibacterial ingredients from Lonicera Japonica Leaves were extracted more fully by the normal reflux, the optimized technology is stable and viable.

Lonicera japonica leaveseffective antibacterial ingredientsextraction processantibacterial ac-tivity

李世传、杜华英、熊建华、汤凯洁、吴国平、吴磊燕

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江西农业大学食品科学与工程学院，江西南昌330045

双胞胎集团研发中心，江西南昌330096

金银花叶 抑菌活性成分 提取工艺 抑菌效果

国家自然科学基金地区基金江西省科技支撑项目

3106022620122BBF60080

2015

10.3969/j.issn.1005-6521.2015.06.002

食品研究与开发
天津市食品研究所,天津市食品工业生产力促进中心

食品研究与开发

CSTPCD北大核心
影响因子：0.561
ISSN：1005-6521
年,卷(期)：2015.(6)
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